Details:

Alternative Title:
Clin Chim Acta
Personal Author:
Dirks, Niek F. ; Vesper, Hubert W. ; van Herwaarden, Antonius E. ; van den Ouweland, Jody M.W. ; Kema, Ido P. ; ... More +
Dirks, Niek F. ; Vesper, Hubert W. ; van Herwaarden, Antonius E. ; van den Ouweland, Jody M.W. ; Kema, Ido P. ; Krabbe, Johannes G. ; Heijboer, Annemieke C. Less -
Description:
Background

The variety of LC-MS/MS methods measuring total 25(OH)D used today is vast and the comparability among these methods is still not well assessed.

Methods

Here, we performed a comparison in samples of healthy donors between the currently routinely used 25(OH)D LC-MS/MS methods in the Netherlands and the Ghent University reference measurement procedure to address this issue (n = 40). Additionally, an interlaboratory comparison in patient serum samples assessed agreement between the Dutch diagnostic methods (n = 37).

Results

The overall correlation of the routine methods for 25(OH)D3 with the reference measurement procedures and with the mean of all diagnostic methods was excellent (r > 0.993 and r > 0.989, respectively). Three out of five methods aligned perfectly with both the reference measurement procedure and the median of all methods. One of the routine methods showed a small positive bias, while another showed a small negative bias consistently in both comparisons.

Conclusion

The biases most probably originated from differences in calibration procedure and may be obviated by reassessing calibration of stock standards and/or calibrator matrices. In conclusion, five diagnostic centers have performed a comparison with the 25(OH)D Ghent University reference measurement procedure in healthy donor serum samples and a comparison among themselves in patient serum samples. Both analyses showed a high correlation and specificity of the routine LC-MS/MS methods, yet did reveal some small standardization issues that could not be traced back to the technical details of the different methods. Hence, this study indicates various calibration procedures can result in perfect alignment.


Subjects:
[+]
25-Hydroxyvitamin D 2 Article Calcifediol Calibration Chromatography, Liquid Healthy Volunteers Humans Mass Spectrometry Method Comparison Standardization Tandem Mass Spectrometry Vitamin D
Source:
Clin Chim Acta. 462:49-54.
Pubmed ID:
27570062
Pubmed Central ID:
PMC5703036
Document Type:
Journal Article
Funding:
CC999999/Intramural CDC HHS/United States
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:da065e267813e778338a411d6f02fc92190c3db56161e938aa4603a633fc6163
Download URL:
https://stacks.cdc.gov/view/cdc/50392/cdc_50392_DS1.pdf
File Type:

Supporting Files

• 	NIHMS920473-supplement-Fig_S1_S2.docx	docx
  	nihms920473.nxml	xml
  	nihms920473f1.gif	gif
  	nihms920473f1.jpg	jpeg
  	nihms920473f2.gif	gif
  	nihms920473f2.jpg	jpeg

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