Alcohol Inhibits Organic Dust-induced ICAM-1 Expression on Bronchial Epithelial Cells

Details

• Alternative Title:
  Safety (Basel)
• Personal Author:
  Wyatt, Todd A. ; Canady, Kerry ; Heires, Art J. ; Poole, Jill A. ; Bailey, Kristina L. ; Nordgren, Tara M. ; Romberger, Debra J.
• Description:
  Methods
  
  Bronchial epithelial cells (BEAS-2B) and primary human bronchial epithelial cells were pretreated with ethanol (EtOH) or TACE inhibitor. ICAM-1 surface expression, TNFα release, and TACE activity were analyzed following HDE stimulation. The effect of alcohol and TACE inhibition on HDE-regulated epithelial cell/neutrophil adhesion interactions was investigated. Finally, utilizing an established animal model, C57BL/6 mice were fed ad libitum ethanol (20%) in drinking water for 8 wk followed by daily intranasal inhalation of HDE or saline during the final two weeks. Mice were sacrificed and lung sections immunostained for ICAM-1.
  
  Results
  
  Pretreatment with alcohol or TACE inhibitor significantly decreased HDE-induced ICAM-1 expression and TNFα release. HDE augmented neutrophil adhesion to epithelial cells, which was decreased with alcohol (32% decrease) or TACE inhibitor (55% decrease) pretreatment. TACE activity increased following HDE exposure, but TACE activity was inhibited following alcohol pretreatment. Alcohol-fed mice demonstrated decreased HDE-induced airway epithelium ICAM-1 expression.
  
  Conclusions
  
  Alcohol diminishes HDE-induced ICAM-1 expression, TNFα release, and neutrophil adhesion via inhibition of TACE activity. These results suggest that alcohol may be an important modulator of lung innate immune responses following CAFO exposure.
  
  
• Keywords:
  Alcohol Bronchial Epithelial Cells ICAM-1 Inflammatory Lung Dieseas Organic Dust
• Source:
  Safety (Basel). 3(1)
• Pubmed ID:
  29082234
• Pubmed Central ID:
  PMC5658133
• Document Type:
  Journal Article
• Funding:
  IK6 BX003781/BX/BLRD VAUnited States/ ; R01 AA017993/AA/NIAAA NIH HHSUnited States/ ; U54OH010162/ACL/ACL HHSUnited States/ ; R01 OH008539/OH/NIOSH CDC HHSUnited States/ ; R01 AG053553/AG/NIA NIH HHSUnited States/ ; R01 ES019325/ES/NIEHS NIH HHSUnited States/ ; U54 OH010162/OH/NIOSH CDC HHSUnited States/
• Volume:
  3
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:dcbdc76c5fbb5bfdfbdaaec3cd936b14cc1290d6ad1311e060efb5f850591a9a
• Download URL:
  https://stacks.cdc.gov/view/cdc/49541/cdc_49541_DS1.pdf
• File Type:
  [PDF - 3.13 MB ]