Measurement of Urinary Benzo[a]pyrene Tetrols and Their Relationship to Other Polycyclic Aromatic Hydrocarbon Metabolites and Cotinine in Humans

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• Alternative Title:
  Chemosphere
• Personal Author:
  Hilton, Donald C. ; Trinidad, Debra A. ; Hubbard, Kendra ; Li, Zheng ; Sjödin, Andreas
• Description:
  Biomonitoring of exposure to polycyclic aromatic hydrocarbons (PAHs) typically uses measurement of metabolites of PAHs with four or less aromatic rings, such as 1-hydroxypyrene, even though interest may be in exposure to larger and carcinogenic PAHs, such as benzo[a]pyrene (B[a]P). An improved procedure for measuring two tetrol metabolites of B[a]P has been developed. Using 2 mL urine, the method includes enzymatic deconjugation of the tetrol conjugates, liquid-liquid extraction, activated carbon solid phase extraction (SPE) and Strata-X SPE, and gas chromatography-electron capture negative ionization-tandem mass spectrometric determination. Limits of detection were 0.026 pg/mL (benzo[a]pyrene-r-7,t-8,t-9,c-10-tetrahydrotetrol, BPT I-1) and 0.090 pg/mL (benzo[a]pyrene-r-7,t-8,c-9,c-10-tetrahydrotetrol, BPT II-1). We quantified BPT I-1 and BPT II-1 in urine from a volunteer who consumed one meal containing high levels of PAHs (barbequed chicken). We also measured urinary concentrations of BPT I-1 and BPT II-1 in smokers and nonsmokers, and compared these concentrations with those of monohydroxy PAHs (OH-PAHs) and cotinine. Urinary elimination of BPT I-1 and BPT II-1 as a function of time after dietary exposure was similar to that observed previously for OH-PAHs. While the median BPT I-1 concentration in smokers' urine (0.069 pg/mL) significantly differs from nonsmokers (0.043 pg/mL), BPT I-1 is only weakly correlated with cotinine. The urinary concentration of BPT I-1 shows a weaker relationship to tobacco smoke than metabolites of smaller PAHs, suggesting that other routes of exposure such as for example dietary routes may be of larger quantitative importance.
• Subjects:
  1-Hydroxypyrene Article Benzo(a)pyrene Benzo[a]pyrene Cotinine Environmental Exposure Environmental Monitoring Gas Chromatography-Mass Spectrometry Humans Methylnaphthalene PAH Polycyclic Aromatic Hydrocarbons Pyrenes Tobacco Smoke

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• Source:
  Chemosphere. 189:365-372.
• Pubmed ID:
  28946070
• Pubmed Central ID:
  PMC5647254
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHS/United States
• Volume:
  189
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:4cf240dfb2fe03e8a062bbb5219f5145e549f3495698d021cf0284677ac39a86
• Download URL:
  https://stacks.cdc.gov/view/cdc/49241/cdc_49241_DS1.pdf
• File Type:
  [PDF - 1.11 MB ]