Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Gymoese, Pernille ; Sørensen, Gitte ; Litrup, Eva ; Olsen, John Elmerdal ; Nielsen, Eva Møller ; ... More +
Gymoese, Pernille ; Sørensen, Gitte ; Litrup, Eva ; Olsen, John Elmerdal ; Nielsen, Eva Møller ; Torpdahl, Mia Less -
Description:
Whole-genome sequencing is rapidly replacing current molecular typing methods for surveillance purposes. Our study evaluates core-genome single-nucleotide polymorphism analysis for outbreak detection and linking of sources of Salmonella enterica serovar Typhimurium and its monophasic variants during a 7-month surveillance period in Denmark. We reanalyzed and defined 8 previously characterized outbreaks from the phylogenetic relatedness of the isolates, epidemiologic data, and food traceback investigations. All outbreaks were identified, and we were able to exclude unrelated and include additional related human cases. We were furthermore able to link possible food and veterinary sources to the outbreaks. Isolates clustered according to sequence types (STs) 19, 34, and 36. Our study shows that core-genome single-nucleotide polymorphism analysis is suitable for surveillance and outbreak investigation for Salmonella Typhimurium (ST19 and ST36), but whole genome-wide analysis may be required for the tight genetic clone of monophasic variants (ST34).
Subject:
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Bacteria
Denmark
Foodborne Disease
Food Safety
Genomics
Investigation Of Outbreaks Of Salmonella Enterica Serovar Typhimurium And Its Monophasic Variants Using Whole-Genome Sequencing, Denmark
MLVA
Molecular Typing
Monophasic Variants
Multilocus Variable-number Tandem-repeat Analysis
Outbreaks
Research
Salmonella Typhimurium
Sequence Analysis
Single-nucleotide Polymorphism
SNP
Surveillance
Whole-genome Sequencing

Source:
Emerg Infect Dis. 23(10):1631-1639.
Pubmed ID:
28930002
Pubmed Central ID:
PMC5621559
Document Type:
Journal Article
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:6c6fcb41b4b36670178932bc1b76eebf7ec4d3c1ef5b75ce1e1abe62c1b4ea07
File Type:

Supporting Files

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  	16-1248-Techapp-s2.pdf	pdf
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