Investigation of Outbreaks of Salmonella enterica Serovar Typhimurium and Its Monophasic Variants Using Whole-Genome Sequencing, Denmark

Gymoese, Pernille; Sørensen, Gitte; Litrup, Eva; Olsen, John Elmerdal; Nielsen, Eva Møller; Torpdahl, Mia

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

Investigation of Outbreaks of Salmonella enterica Serovar Typhimurium and Its Monophasic Variants Using Whole-Genome Sequencing, Denmark

Published Date:

Oct 2017

Source:

Emerg Infect Dis. 23(10):1631-1639.

[PDF-2.25 MB]

Details:

Alternative Title:

Emerg Infect Dis

Personal Author:

Gymoese, Pernille ; Sørensen, Gitte ; Litrup, Eva ; Olsen, John Elmerdal ; Nielsen, Eva Møller ; ... More ▼

Description:

Whole-genome sequencing is rapidly replacing current molecular typing methods for surveillance purposes. Our study evaluates core-genome single-nucleotide polymorphism analysis for outbreak detection and linking of sources of Salmonella enterica serovar Typhimurium and its monophasic variants during a 7-month surveillance period in Denmark. We reanalyzed and defined 8 previously characterized outbreaks from the phylogenetic relatedness of the isolates, epidemiologic data, and food traceback investigations. All outbreaks were identified, and we were able to exclude unrelated and include additional related human cases. We were furthermore able to link possible food and veterinary sources to the outbreaks. Isolates clustered according to sequence types (STs) 19, 34, and 36. Our study shows that core-genome single-nucleotide polymorphism analysis is suitable for surveillance and outbreak investigation for Salmonella Typhimurium (ST19 and ST36), but whole genome-wide analysis may be required for the tight genetic clone of monophasic variants (ST34).

Subject:

[+]

Pubmed ID:

28930002

Pubmed Central ID:

PMC5621559

Document Type:

Journal Article

Collection(s):

Emerging Infectious Diseases

Main Document Checksum:

[+]

Supporting Files:

	16-1248-Techapp-s1.xlsx	application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
	16-1248-Techapp-s2.pdf	application/pdf
	16-1248.nxml	text/xml
	16-1248-F1.gif	image/gif
	16-1248-F1.jpg	image/jpeg
	16-1248-F2.gif	image/gif
	16-1248-F2.jpg	image/jpeg
	16-1248-F3.gif	image/gif
	16-1248-F3.jpg	image/jpeg
	16-1248-F4.gif	image/gif
	16-1248-F4.jpg	image/jpeg

No Related Documents.

You May Also Like:

Microevolution of Monophasic Salmonella Typhimurium during Epidemic, United Kingdom, 2005–2010

Personal Author:

Petrovska, Liljana; Mather, Alison E.; AbuOun, Manal; Branchu, Priscilla; Harris, Simon R.; Connor, Thomas; Hopkins, K.L.; Underwood, A.; Lettini, Antonia A.; Page, Andrew; Bagnall, Mary; Wain, John; Parkhill, Julian; Dougan, Gordon; Davies, Robert; Kingsley, Robert A.;

Published Date:

Apr 2016

Source:

Emerg Infect Dis. 22(4):617-624.

Description:

Microevolution associated with emergence and expansion of new epidemic clones of bacterial pathogens holds the key to epidemiologic success. To determine microevolution associated with monophasic Salmonella Typhimurium during an epidemic, we performe...

File Type:

[PDF - 685.84 KB]
Genomic Analysis of Salmonella enterica Serovar Typhimurium DT160 Associated with a 14-Year Outbreak, New Zealand, 1998–2012

Personal Author:

Bloomfield, Samuel J.; Benschop, Jackie; Biggs, Patrick J.; Marshall, Jonathan C.; Hayman, David T.S.; Carter, Philip E.; Midwinter, Anne C.; Mather, Alison E.; French, Nigel P.;

Published Date:

Jun 2017

Source:

Emerg Infect Dis. 23(6):906-913.

Description:

During 1998-2012, an extended outbreak of Salmonella enterica serovar Typhimurium definitive type 160 (DT160) affected >3,000 humans and killed wild birds in New Zealand. However, the relationship between DT160 within these 2 host groups and the orig...

File Type:

[PDF - 1.69 MB]
Zoonotic Source Attribution of Salmonella enterica Serotype Typhimurium Using Genomic Surveillance Data, United States

Personal Author:

Zhang, Shaokang; Li, Shaoting; Gu, Weidong; den Bakker, Henk; Boxrud, Dave; Taylor, Angie; Roe, Chandler; Driebe, Elizabeth; Engelthaler, David M.; Allard, Marc; Brown, Eric; McDermott, Patrick; Zhao, Shaohua; Bruce, Beau B.; Trees, Eija; Fields, Patricia I.; Deng, Xiangyu;

Published Date:

January 2019

Source:

Emerg Infect Dis. 25(1):82-91

Description:

Increasingly, routine surveillance and monitoring of foodborne pathogens using whole-genome sequencing is creating opportunities to study foodborne illness epidemiology beyond routine outbreak investigations and case-control studies. Using a global p...

File Type:

[PDF - 3.42 MB]
Foodborne Outbreak and Nonmotile Salmonella enterica Variant, France

Personal Author:

Le Hello, Simon; Brisabois, Anne; Accou-Demartin, Marie; Josse, Adeline; Marault, Muriel; Francart, Sylvie; Da Silva, Nathalie Jourdan; Weill, François-Xavier;

Published Date:

Jan 2012

Source:

Emerg Infect Dis. 18(1):132-134.

Description:

We report a food-related outbreak of salmonellosis in humans caused by a nonmotile variant of Salmonella enterica serotype Typhimurium in France in 2009. This nonmotile variant had been circulating in laying hens but was not considered as Typhimurium...

File Type:

[PDF - 171.06 KB]
Meta-Analysis of Pulsed-Field Gel Electrophoresis Fingerprints Based on a Constructed Salmonella Database

Personal Author:

Zou, Wen; Chen, Hung-Chia; Hise, Kelley B.; Tang, Hailin; Foley, Steven L.; Meehan, Joe; Lin, Wei-Jiun; Nayak, Rajesh; Xu, Joshua; Fang, Hong; Chen, James J.;

Published Date:

Mar 14 2013

Source:

PLoS One. 2013; 8(3).

Description:

A database was constructed consisting of 45,923 Salmonella pulsed-field gel electrophoresis (PFGE) patterns. The patterns, randomly selected from all submissions to CDC PulseNet during 2005 to 2010, included the 20 most frequent serotypes and 12 less...

File Type:

[PDF - 953.05 KB]