Differential Pulmonary Effects of Wintertime California and China Particulate Matter in Healthy Young Mice

Details

• Alternative Title:
  Toxicol Lett
• Personal Author:
  Sun, Xiaolin ; Wei, Haiying ; Young, Dominique E. ; Bein, Keith J. ; Smiley-Jewell, Suzette M. ; Zhang, Qi ; Fulgar, Ciara Catherine B. ; Castañeda, Alejandro R. ; Pham, Alexa K. ; Li, Wei ; Pinkerton, Kent E.
• Description:
  Airborne particulate matter (PM) is associated with adverse cardiorespiratory effects. To better understand source-orientated PM toxicity, a comparative study of the biological effects of fine PM (diameter≤2.5μm, PM|) collected during the winter season from Shanxi Province, China, and the Central Valley, California, United States, was conducted. The overarching hypothesis for this study was to test whether the chemical composition of PM on an equal mass basis from two urban areas, one in China and one in California, can lead to significantly different effects of acute toxicity and inflammation in the lungs of healthy young mice. Male, 8-week old BALB/C mice received a single 50μg dose of vehicle, Taiyuan PM or Sacramento PM by oropharyngeal aspiration and were sacrificed 24h later. Bronchoalveolar lavage, ELISA and histopathology were performed along with chemical analysis of PM composition. Sacramento PM had a greater proportion of oxidized organic material, significantly increased neutrophil numbers and elevated CXCL-1 and TNF-α protein levels compared to the Taiyuan PM. The findings suggest that Sacramento PM| was associated with a greater inflammatory response compared to that of Taiyuan PM| that may be due to a higher oxidice. Male, 8-week old BALB/C mice received a single 50μg dose of vehicle, Taiyuan PM or Sacramento PM by oropharyngeal aspiration and were sacrificed 24h later. Bronchoalveolar lavage, ELISA and histopathology were performed along with chemical analysis of PM composition. Sacramento PM had a greater proportion of oxidized organic material, significantly increased neutrophil numbers and elevated CXCL-1 and TNF-α protein levels compared to the Taiyuan PM. The findings suggest that Sacramento PM| was associated with a greater inflammatory response compared to that of Taiyuan PM| that may be due to a higher oxidized state of organic carbon and copper content.
• Subjects:
  Animals Bronchoalveolar Lavage Fluid Chemokine CXCL1 Enzyme-Linked Immunosorbent Assay Inflammation Mediators Inhalation Exposure Lung Male Mass Spectrometry Mice, Inbred BALB C Neutrophil Infiltration Neutrophils Oxidative Stress Particle Size Particulate Matter Pneumonia Seasons Time Factors Tumor Necrosis Factor-alpha

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• Source:
  Toxicol Lett. 278:1-8
• Pubmed ID:
  28698096
• Pubmed Central ID:
  PMC5572813
• Document Type:
  Journal Article
• Funding:
  P30 ES023513/ES/NIEHS NIH HHSUnited States/ ; T32 GM099608/GM/NIGMS NIH HHSUnited States/ ; T32 HL007013/HL/NHLBI NIH HHSUnited States/ ; U54 OH007550/OH/NIOSH CDC HHSUnited States/
• Place as Subject:
  California ; China
• Volume:
  278
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:b78e1ad8b8e7c65e7b72cecbb05c57b9b075521cf2941f9e8a96d7615fce03a2
• Download URL:
  https://stacks.cdc.gov/view/cdc/47801/cdc_47801_DS1.pdf
• File Type:
  [PDF - 1.91 MB ]