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Induction of Stem Cell-derived Functional Neurons via NanoScript-based Gene Repression
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10 05 2015
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Source: Angew Chem Int Ed Engl. 54(41):11983-11988
[PDF-938.45 KB]
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Alternative Title:Angew Chem Int Ed Engl
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Description:Even though gene repression is a powerful approach to exogenously regulate cellular behavior, developing a platform to effectively repress targeted genes, especially for stem-cell applications, remains elusive. Herein, we introduce a nanomaterial-based platform that is capable of mimicking the function of transcription repressor proteins to downregulate gene expression at the transcriptional level for enhancing stem-cell differentiation. We developed the "NanoScript" platform by integrating multiple gene repression molecules with a nanoparticle. First, we show a proof-of-concept demonstration using a GFP-specific NanoScript to knockdown GFP expression in neural stem cells (NSCs-GFP). Then, we show that a Sox9-specific NanoScript can repress Sox9 expression to initiate enhanced differentiation of NSCs into functional neurons. Overall, the tunable properties and gene-knockdown capabilities of NanoScript enables its utilization for gene-repression applications in stem cell biology.
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Pubmed ID:26292201
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Pubmed Central ID:PMC5568028
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Volume:54
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Issue:41
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