i
Induction of Stem Cell-derived Functional Neurons via NanoScript-based Gene Repression
-
10 05 2015
-
-
Source: Angew Chem Int Ed Engl. 54(41):11983-11988
[PDF-938.45 KB]
Details:
-
Alternative Title:Angew Chem Int Ed Engl
-
Personal Author:
-
Description:Even though gene repression is a powerful approach to exogenously regulate cellular behavior, developing a platform to effectively repress targeted genes, especially for stem-cell applications, remains elusive. Herein, we introduce a nanomaterial-based platform that is capable of mimicking the function of transcription repressor proteins to downregulate gene expression at the transcriptional level for enhancing stem-cell differentiation. We developed the "NanoScript" platform by integrating multiple gene repression molecules with a nanoparticle. First, we show a proof-of-concept demonstration using a GFP-specific NanoScript to knockdown GFP expression in neural stem cells (NSCs-GFP). Then, we show that a Sox9-specific NanoScript can repress Sox9 expression to initiate enhanced differentiation of NSCs into functional neurons. Overall, the tunable properties and gene-knockdown capabilities of NanoScript enables its utilization for gene-repression applications in stem cell biology.
-
Keywords:
-
Source:
-
Pubmed ID:26292201
-
Pubmed Central ID:PMC5568028
-
Document Type:
-
Funding:
-
Collection(s):
-
Main Document Checksum:
-
Download URL:
-
File Type:
