Induction of Stem Cell-derived Functional Neurons via NanoScript-based Gene Repression

Patel, Sahishnu; Chueng, Sy-Tsong Dean; Yin, Perry T.; Dardir, Kholud; Song, Zhichao; Pasquale, Nicholas; Kwan, Kelvin; Sugiyama, Hiroshi; Lee, Ki-Bum

doi:10.1002/anie.201504902

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Induction of Stem Cell-derived Functional Neurons via NanoScript-based Gene Repression

Published Date:

Aug 20 2015

Publisher's site:

http://dx.doi.org/10.1002/anie.201504902

Source:

Angew Chem Int Ed Engl. 54(41):11983-11988.

[PDF-938.45 KB]

Details:

Alternative Title:

Angew Chem Int Ed Engl

Personal Author:

Patel, Sahishnu ; Chueng, Sy-Tsong Dean ; Yin, Perry T. ; Dardir, Kholud ; Song, Zhichao ; ... More ▼

Description:

Even though gene repression is a powerful approach to exogenously regulate cellular behavior, developing a platform to effectively repress targeted genes, especially for stem-cell applications, remains elusive. Herein, we introduce a nanomaterial-based platform that is capable of mimicking the function of transcription repressor proteins to downregulate gene expression at the transcriptional level for enhancing stem-cell differentiation. We developed the "NanoScript" platform by integrating multiple gene repression molecules with a nanoparticle. First, we show a proof-of-concept demonstration using a GFP-specific NanoScript to knockdown GFP expression in neural stem cells (NSCs-GFP). Then, we show that a Sox9-specific NanoScript can repress Sox9 expression to initiate enhanced differentiation of NSCs into functional neurons. Overall, the tunable properties and gene-knockdown capabilities of NanoScript enables its utilization for gene-repression applications in stem cell biology.

Subject:

[+]

Pubmed ID:

26292201

Pubmed Central ID:

PMC5568028

Document Type:

Journal Article

Funding:

R01 DC015000/DC/NIDCD NIH HHS/United States ; R21 NS085569/NS/NINDS NIH HHS/United States ; 1DP20D006462-01/DP/NCCDPHP CDC HHS/United States ; 1R21NS085569-01/NS/NINDS NIH HHS/United States ;

Collection(s):

CDC Public Access

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Induction of Stem Cell-derived Functional Neurons via NanoScript-based Gene Repression

Induction of Stem Cell-derived Functional Neurons via NanoScript-based Gene Repression

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