Details:

Alternative Title:
Angew Chem Int Ed Engl
Publisher's site:
http://dx.doi.org/10.1002/anie.201504902
Personal Author:
Patel, Sahishnu ; Chueng, Sy-Tsong Dean ; Yin, Perry T. ; Dardir, Kholud ; Song, Zhichao ; ... More +
Patel, Sahishnu ; Chueng, Sy-Tsong Dean ; Yin, Perry T. ; Dardir, Kholud ; Song, Zhichao ; Pasquale, Nicholas ; Kwan, Kelvin ; Sugiyama, Hiroshi ; Lee, Ki-Bum Less -
Description:
Even though gene repression is a powerful approach to exogenously regulate cellular behavior, developing a platform to effectively repress targeted genes, especially for stem-cell applications, remains elusive. Herein, we introduce a nanomaterial-based platform that is capable of mimicking the function of transcription repressor proteins to downregulate gene expression at the transcriptional level for enhancing stem-cell differentiation. We developed the "NanoScript" platform by integrating multiple gene repression molecules with a nanoparticle. First, we show a proof-of-concept demonstration using a GFP-specific NanoScript to knockdown GFP expression in neural stem cells (NSCs-GFP). Then, we show that a Sox9-specific NanoScript can repress Sox9 expression to initiate enhanced differentiation of NSCs into functional neurons. Overall, the tunable properties and gene-knockdown capabilities of NanoScript enables its utilization for gene-repression applications in stem cell biology.
Subjects:
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Article Biomimetic Materials Biomimetics Gene Expression Gene Knockdown Gene Knockdown Techniques Gene Regulation Green Fluorescent Proteins Humans Nanoparticles Neural Stem Cells Neurogenesis Neuronal Differentiation Neurons Nylons Promoter Regions, Genetic Repressor Proteins SOX9 Transcription Factor Transcription Repressor Proteins
Source:
Angew Chem Int Ed Engl. 54(41):11983-11988.
Pubmed ID:
26292201
Pubmed Central ID:
PMC5568028
Document Type:
Journal Article
Funding:
R01 DC015000/DC/NIDCD NIH HHS/United States ; R21 NS085569/NS/NINDS NIH HHS/United States ; 1DP20D006462-01/DP/NCCDPHP CDC HHS/United States ; 1R21NS085569-01/NS/NINDS NIH HHS/United States
R01 DC015000/DC/NIDCD NIH HHS/United States ; R21 NS085569/NS/NINDS NIH HHS/United States ; 1DP20D006462-01/DP/NCCDPHP CDC HHS/United States ; 1R21NS085569-01/NS/NINDS NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:84d16e599938c5ba3bf653e217d7a7eb06f01441c0700f2f330b70b5b2645881
Download URL:
https://stacks.cdc.gov/view/cdc/47731/cdc_47731_DS1.pdf
File Type:

Supporting Files

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  	NIHMS753161-supplement-Supporting_Information.pdf	pdf
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