Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Charpentier, Elena ; Garnaud, Cécile ; Wintenberger, Claire ; Bailly, Sébastien ; Murat, Jean-Benjamin ; ... More +
Charpentier, Elena ; Garnaud, Cécile ; Wintenberger, Claire ; Bailly, Sébastien ; Murat, Jean-Benjamin ; Rendu, John ; Pavese, Patricia ; Drouet, Thibault ; Augier, Caroline ; Malvezzi, Paolo ; Thiébaut-Bertrand, Anne ; Mallaret, Marie-Reine ; Epaulard, Olivier ; Cornet, Muriel ; Larrat, Sylvie ; Maubon, Danièle Less -
Description:
Pneumocystis jirovecii is a major threat for immunocompromised patients, and clusters of pneumocystis pneumonia (PCP) have been increasingly described in transplant units during the past decade. Exploring an outbreak transmission network requires complementary spatiotemporal and strain-typing approaches. We analyzed a PCP outbreak and demonstrated the added value of next-generation sequencing (NGS) for the multilocus sequence typing (MLST) study of P. jirovecii strains. Thirty-two PCP patients were included. Among the 12 solid organ transplant patients, 5 shared a major and unique genotype that was also found as a minor strain in a sixth patient. A transmission map analysis strengthened the suspicion of nosocomial acquisition of this strain for the 6 patients. NGS-MLST enables accurate determination of subpopulation, which allowed excluding other patients from the transmission network. NGS-MLST genotyping approach was essential to deciphering this outbreak. This innovative approach brings new insights for future epidemiologic studies on this uncultivable opportunistic fungus.
Subject:
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Added Value Of Next-Generation Sequencing For Multilocus Sequence Typing Analysis Of A Pneumocystis Jirovecii Pneumonia Outbreak
Disease Outbreaks
Fungi
High-throughput Nucleotide Sequencing
MLST
Multilocus Sequence Typing
Next-generation Sequencing
NGS
Pneumocystis Jirovecii
Pneumocystosis
Pneumonia
Respiratory Infections
Synopsis
Transplants

Source:
Emerg Infect Dis. 23(8):1237-1245.
Pubmed ID:
28726611
Pubmed Central ID:
PMC5547796
Document Type:
Journal Article
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
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urn:sha256:36bcc1ba5dec657871e33442f0840cb7a3d0b39e9415f4d40f7c927f894cf4e8
File Type:

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