Size-Dependent Deposition, Translocation, and Microglial Activation of Inhaled Silver Nanoparticles in the Rodent Nose and Brain

Details

• Journal Article:
  Environmental Health Perspectives
• Personal Author:
  Patchin, Esther S. ; Anderson, Donald S. ; Silva, Rona M. ; Uyeminami, Dale L. ; Scott, Grace M. ; Guo, Ting ; Van Winkle, Laura S. ; Pinkerton, Kent E.
• Description:
  Background: Silver nanoparticles (AgNP) are present in personal, commercial, and industrial products, which are often aerosolized. Current understanding of the deposition, translocation, and health-related impacts of AgNP inhalation is limited. Objectives: To determine 1) the deposition and retention of inhaled Ag in the nasal cavity from nose-only exposure; 2) the timing for Ag translocation to and retention/clearance in the olfactory bulb (OB); and 3) whether the presence of Ag in the OB affects microglial activity. Methods: Male Sprague-Dawley rats were exposed nose-only to citrate-buffered 20 or 110 nm AgNP (C20 or C110, respectively) or citrate buffer alone for six hours. The nasal cavity and OB were examined for the presence of Ag and biological responses up to 56 days post exposure (8 weeks). Results: The highest nasal Ag deposition was observed at Day 0 for both AgNP sizes. Aerosolized C20 resulted in rapid translocation of Ag to the OB and microglial activation at Days 0, 1 and 7. In contrast, inhalation of C110 resulted in a gradual but progressive transport of Ag to and retention in the OB, with a trend for microglial activation to variably be above control. Conclusions: This study demonstrates that after a six-hour inhalation exposure in rats to 20 and 110 nm AgNP at a single point in time, Ag deposition in the nose, the rate of translocation to the brain, and subsequent microglial activation in the OB, differed depending on AgNP size and time since exposure. [Description provided by NIOSH]
• Subjects:
  Aerosols Laboratories Nanoparticles Nose Occupational Health Respiratory System Safety Sense Organs Silver
• Keywords:
  Silver compounds; Nanomaterials; Nanotechnology; Aerosols; Inhalation; Nasal cavity; Olfactory system; Laboratory animals; Brain matter; Brain function
• Source:
  Environ Health Perspect 2016 Dec; 124(12):1870-1875
• ISSN:
  0091-6765
• Pubmed ID:
  27152509
• Pubmed Central ID:
  PMC5132640
• Document Type:
  Text
• Funding:
  U50 OH007550/OH/NIOSH CDC HHS/United States ; T32 HL086350/HL/NHLBI NIH HHS/United States ; P42 ES004699/ES/NIEHS NIH HHS/United States ; P30 ES023513/ES/NIEHS NIH HHS/United States ; U01 ES020127/ES/NIEHS NIH HHS/United States ; U54 OH007550/OH/NIOSH CDC HHS/United States
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  6 pdf pages
• Volume:
  124
• Issue:
  12
• NIOSHTIC Number:
  nn:20047987
• Contact Point Address:
  Kent E. Pinkerton, Center for Health and the Environment, University of California, One Shields Avenue, Davis, CA 95616, USA
• Email:
  kepinkerton@ucdavis.edu
• CAS Registry Number:
  Silver (CAS RN 7440-22-4)
• Federal Fiscal Year:
  2017
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Collection(s):
  National Institute for Occupational Safety and Health CDC Public Access
• Main Document Checksum:
  urn:sha-512:80f609d077ea1c6b95b5168288e6fa676e9956d35ebb81d5720c4e9a8c713dd2b20ca3d6508f4bf54fb21f76eb022ac2b392827f10534855487cf18ff5450800
• Download URL:
  https://stacks.cdc.gov/view/cdc/44867/cdc_44867_DS1.pdf
• File Type:
  [PDF - 403.67 KB ]