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A new dual-collimation batch reactor for determination of ultraviolet inactivation rate constants for microorganisms in aqueous suspensions
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Jul 25 2016
Source: J Photochem Photobiol B. 162:674-680. -
Alternative Title:J Photochem Photobiol B
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Description:We developed, characterized, and tested a new dual-collimation aqueous UV reactor to improve the accuracy and consistency of aqueous k-value determinations. This new system is unique because it collimates UV energy from a single lamp in two opposite directions. The design provides two distinct advantages over traditional single-collimation systems: 1) real-time UV dose (fluence) determination; and 2) simple actinometric determination of a reactor factor that relates measured irradiance levels to actual irradiance levels experienced by the microbial suspension. This reactor factor replaces three of the four typical correction factors required for single-collimation reactors. Using this dual-collimation reactor, Bacillus subtilis spores demonstrated inactivation following the classic multi-hit model with k=0.1471cm(2)/mJ (with 95% confidence bounds of 0.1426 to 0.1516).
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Pubmed ID:27498232
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Pubmed Central ID:PMC5362826
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