A new dual-collimation batch reactor for determination of ultraviolet inactivation rate constants for microorganisms in aqueous suspensions

Details

• Personal Author:
  Bahnfleth WP ; Blum DH ; Freihaut JD ; Kremer PA ; Martin SB Jr. ; Schauer ES
• Description:
  We developed, characterized, and tested a new dual-collimation aqueous UV reactor to improve the accuracy and consistency of aqueous k-value determinations. This new system is unique because it collimates UV energy from a single lamp in two opposite directions. The design provides two distinct advantages over traditional single-collimation systems: 1) real-time UV dose (fluence) determination; and 2) simple actinometric determination of a reactor factor that relates measured irradiance levels to actual irradiance levels experienced by the microbial suspension. This reactor factor replaces three of the four typical correction factors required for single-collimation reactors. Using this dual-collimation reactor, Bacillus subtilis spores demonstrated inactivation following the classic multi-hit model with k = 0.1471 cm2/mJ (with 95% confidence bounds of 0.1426 to 0.1516). [Description provided by NIOSH]
• Subjects:
  Fungi Microbiology Occupational Health Safety Ultraviolet Rays
• Keywords:
  Aqueous-solutions Author Keywords: Ultraviolet Bacillus Subtilis Bacteria Collimated-beam Reactor Germicidal Inactivation Rate Constant K-Value Microbial-test-systems Microorganisms Reaction-rates Ultraviolet-light

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• ISSN:
  1011-1344
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Massachusetts ; OSHA Region 1 ; OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  RHD - Respiratory Health Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  162
• NIOSHTIC Number:
  nn:20048461
• Citation:
  J Photochem Photobiol B 2016 Sep; 162:674-680
• Contact Point Address:
  Stephen B. Martin Jr., Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Respiratory Health Division, Field Studies Branch, 1095 Willowdale Road, Morgantown, WV 26505, USA
• Email:
  SMartin1@cdc.gov
• Federal Fiscal Year:
  2016
• NORA Priority Area:
  Healthcare and Social Assistance
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Photochemistry and Photobiology B: Biology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3ffdbe8034fb7a741a7b9380c5d562917889e09bc4c3a51ed657a19380fa95b22b8de284401945d2e20cc08dab3b2e4445d3b43c779c4252b3040dda06c7405e
• Download URL:
  https://stacks.cdc.gov/view/cdc/203211/cdc_203211_DS1.pdf
• File Type:
  [PDF - 999.49 KB ]