Evaluation of Burkholderia cepacia Complex Bacteria Pathogenicity Using Caenorhabditis elegans

Details

• Alternative Title:
  Bio Protoc
• Personal Author:
  Tedesco, Pietro ; Di Schiavi, Elia ; Esposito, Fortunato Palma ; de Pascale, Donatella
• Description:
  This protocol describes two biological assays to evaluate pathogenicity of | complex (Bcc) strains against the nematode | Specifically, these two assays allow one to identify if the under-investigated Bcc strains are able to kill the nematodes by intestinal colonization (slow killing assay, SKA) or by toxins production (fast killing assay, FKA). The principal differences between the two assays rely on the different killing kinetics for worms.
• Subjects:
  • Article
• Source:
  Bio Protoc. 6(20).
• Pubmed ID:
  28255573
• Pubmed Central ID:
  PMC5330310
• Document Type:
  Journal Article
• Funding:
  P40 OD010440/OD/NIH HHS/United States ; U66 IP000114/IP/NCIRD CDC HHS/United States
• Volume:
  6
• Issue:
  20
• Download URL:
  https://stacks.cdc.gov/view/cdc/44632/cdc_44632_DS1.pdf
• File Type:
  [PDF - 102.28 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:7000cf663741cdd0cf3d1edd3b30ff25d0652c7499e3c01aa82a34abb51291c1