Details:

Alternative Title:
PLoS One
Publisher's site:
http://dx.doi.org/10.1371/journal.pone.0172139
Personal Author:
Rogier, Eric ; Plucinski, Mateusz ; Lucchi, Naomi ; Mace, Kimberly ; Chang, Michelle ; ... More +
Rogier, Eric ; Plucinski, Mateusz ; Lucchi, Naomi ; Mace, Kimberly ; Chang, Michelle ; Lemoine, Jean Frantz ; Candrinho, Baltazar ; Colborn, James ; Dimbu, Rafael ; Fortes, Filomeno ; Udhayakumar, Venkatachalam ; Barnwell, John Less -
Description:
Detection of histidine-rich protein 2 (HRP2) from the malaria parasite Plasmodium falciparum provides evidence for active or recent infection, and is utilized for both diagnostic and surveillance purposes, but current laboratory immunoassays for HRP2 are hindered by low sensitivities and high costs. Here we present a new HRP2 immunoassay based on antigen capture through a bead-based system capable of detecting HRP2 at sub-picogram levels. The assay is highly specific and cost-effective, allowing fast processing and screening of large numbers of samples. We utilized the assay to assess results of HRP2-based rapid diagnostic tests (RDTs) in different P. falciparum transmission settings, generating estimates for true performance in the field. Through this method of external validation, HRP2 RDTs were found to perform well in the high-endemic areas of Mozambique and Angola with 86.4% and 73.9% of persons with HRP2 in their blood testing positive by RDTs, respectively, and false-positive rates of 4.3% and 0.5%. However, in the low-endemic setting of Haiti, only 14.5% of persons found to be HRP2 positive by the bead assay were RDT positive. Additionally, 62.5% of Haitians showing a positive RDT test had no detectable HRP2 by the bead assay, likely indicating that these were false positive tests. In addition to RDT validation, HRP2 biomass was assessed for the populations in these different settings, and may provide an additional metric by which to estimate P. falciparum transmission intensity and measure the impact of interventions.
Subjects:
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Adult Aged Aged, 80 And Over Amino Acid Sequence Antigens, Protozoan Child Child, Preschool Cross-Sectional Studies Diagnostic Tests, Routine Endemic Diseases Health Surveys Host-Parasite Interactions Humans Immunoassay Infant Malaria, Falciparum Middle Aged Plasmodium Falciparum Protozoan Proteins Reproducibility Of Results Sensitivity And Specificity Young Adult
Source:
PLoS One. 12(2).
Pubmed ID:
28192523
Pubmed Central ID:
PMC5305216
Document Type:
Journal Article
Place as Subject:
Angola ; Haiti ; Mozambique
Angola ; Haiti ; Mozambique Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:74a43ff2a704d2bbbd92adf3794260d9e9b219f4a49a24f90ab038156e240683
Download URL:
https://stacks.cdc.gov/view/cdc/44413/cdc_44413_DS1.pdf
File Type:

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