Borrelia mayonii sp. nov., a member of the Borrelia burgdorferi sensu lato complex, detected in patients and ticks in the upper midwestern United States

Details

• Alternative Title:
  Int J Syst Evol Microbiol
• Personal Author:
  Pritt, Bobbi S. ; Respicio-Kingry, Laurel B. ; Sloan, Lynne M. ; Schriefer, Martin E. ; Replogle, Adam J. ; Bjork, Jenna ; Liu, Gongping ; Kingry, Luke C. ; Mead, Paul S. ; Neitzel, David F. ; Schiffman, Elizabeth ; Hoang Johnson, Diep K. ; Davis, Jeffrey P. ; Paskewitz, Susan M. ; Boxrud, David ; Deedon, Alecia ; Lee, Xia ; Miller, Tracy K. ; Feist, Michelle A. ; Steward, Christopher R. ; Theel, Elitza S. ; Patel, Robin ; Irish, Cole L. ; Petersen, Jeannine M.
• Description:
  Lyme borreliosis (LB) is a multisystem disease caused by spirochetes in the Borrelia burgdorferisensu lato (Bbsl) genospecies complex. We previously described a novel Bbsl genospecies (type strain MN14-1420T) that causes LB among patients with exposures to ticks in the upper midwestern USA. Patients infected with the novel Bbsl genospecies demonstrated higher levels of spirochetemia and somewhat differing clinical symptoms as compared with those infected with other Bbsl genospecies. The organism was detected from human specimens using PCR, microscopy, serology and culture. The taxonomic status was determined using an eight-housekeeping-gene (uvrA, rplB, recG, pyrG, pepX, clpX, clpA and nifS) multi-locus sequence analysis (MLSA) and comparison of 16S rRNA gene, flaB, rrf-rrl, ospC and oppA2 nucleotide sequences. Using a system threshold of 98.3 % similarity for delineation of Bbsl genospecies by MLSA, we demonstrated that the novel species is a member of the Bbsl genospecies complex, most closely related to B. burgdorferisensu stricto (94.7-94.9 % similarity). This same species was identified in Ixodes scapularis ticks collected in Minnesota and Wisconsin. This novel species, Borrelia mayonii sp. nov, is formally described here. The type strain, MN14-1420, is available through the Deutsche Sammlung von Mikroorganismen und Zelkulturen GmbH (DSM 102811) and the American Type Culture Collection (ATCC BAA-2743).
• Subjects:
  Animals Bacterial Typing Techniques Borrelia Burgdorferi Group DNA, Bacterial Female Genes, Bacterial Humans Ixodes Lyme Disease Phylogeny RNA, Ribosomal, 16S Sequence Analysis, DNA

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• Source:
  Int J Syst Evol Microbiol. 66(11):4878-4880.
• Pubmed ID:
  27558626
• Pubmed Central ID:
  PMC5214957
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHS/United States
• Place as Subject:
  Midwestern United States ; Minnesota ; Wisconsin
• Volume:
  66
• Issue:
  11
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:6efb1389a034a299c105e1c188f9fa11233c7da80305904fd60a2dc69fbb1ec4
• Download URL:
  https://stacks.cdc.gov/view/cdc/43703/cdc_43703_DS1.pdf
• File Type:
  [PDF - 45.56 KB ]