The Gut Microbiota in Conventional and Serrated Precursors of Colorectal Cancer

Details

• Journal Article:
  Microbiome
• Personal Author:
  Peters, Brandilyn A. ; Dominianni, Christine ; Shapiro, Jean A. ; Church, Timothy R. ; Wu, Jing ; Miller, George ; Yuen, Elizabeth ; Freiman, Hal ; Lustbader, Ian ; Salik, James ; Friedlander, Charles ; Hayes, Richard B. ; Ahn, Jiyoung
• Description:
  Background: Colorectal cancer is a heterogeneous disease arising from at least two precursors-the conventional adenoma (CA) and the serrated polyp. We and others have previously shown a relationship between the human gut microbiota and colorectal cancer; however, its relationship to the different early precursors of colorectal cancer is understudied. We tested, for the first time, the relationship of the gut microbiota to specific colorectal polyp types. Results: Gut microbiota were assessed in 540 colonoscopy-screened adults by 16S rRNA gene sequencing of stool samples. Participants were categorized as CA cases (n = 144), serrated polyp cases (n = 73), or polyp-free controls (n = 323). CA cases were further classified as proximal (n = 87) or distal (n = 55) and as non-advanced (n = 121) or advanced (n = 22). Serrated polyp cases were further classified as hyperplastic polyp (HP; n = 40) or sessile serrated adenoma (SSA; n = 33). We compared gut microbiota diversity, overall composition, and normalized taxon abundance among these groups. CA cases had lower species richness in stool than controls (p = 0.03); in particular, this association was strongest for advanced CA cases (p = 0.004). In relation to overall microbiota composition, only distal or advanced CA cases differed significantly from controls (p = 0.02 and p = 0.002). In taxon-based analysis, stool of CA cases was depleted in a network of Clostridia operational taxonomic units from families Ruminococcaceae, Clostridiaceae, and Lachnospiraceae, and enriched in the classes Bacilli and Gammaproteobacteria, order Enterobacteriales, and genera Actinomyces and Streptococcus (all q < 0.10). SSA and HP cases did not differ in diversity or composition from controls, though sample size for these groups was small. Few taxa were differentially abundant between HP cases or SSA cases and controls; among them, class Erysipelotrichi was depleted in SSA cases. Conclusions: Our results indicate that gut microbes may play a role in the early stages of colorectal carcinogenesis through the development of CAs. Findings may have implications for developing colorectal cancer prevention therapies targeting early microbial drivers of colorectal carcinogenesis. An erratum to this article has been published and is available at this link https://doi.org/10.1186/s40168-017-0238-x: Upon publication of this article, it was requested that: "Samples were sequenced at the NYUMC Genome Technology Center. The NYUMC Genome Technology Center is partially supported by the Cancer Center Support Grant, P30CA016087, at the Laura and Isaac Perlmutter Cancer Center" be added to the Acknowledgements section. This acknowledgement has been included by means of this erratum. [Description provided by NIOSH]
• Subjects:
  Adenoma Bacteria Base Sequence Colonoscopy Diet DNA, Bacterial Feces Gastrointestinal Microbiome Hyperplasia Mass Screening Microbiology Microbiota Neoplasms Occupational Health Safety Sequence Analysis, DNA

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• Keywords:
  Author Keywords: Microbiome; Microbiota; Adenoma; Polyp; Colorectal; Cancer; Serrated Colorectal Cancer; Microorganisms; Intestinal Cells; Diet; Digestive System; Bacteria;
• Source:
  Microbiome 2016 Dec; 4(1):69
• ISSN:
  2049-2618
• Pubmed ID:
  28038683
• Pubmed Central ID:
  PMC5203720
• Document Type:
  Text
• Funding:
  R01 CA159036/CA/NCI NIH HHS/United States ; P30 CA016087/CA/NCI NIH HHS/United States ; P30 ES000260/ES/NIEHS NIH HHS/United States ; R21 CA183887/CA/NCI NIH HHS/United States ; R03 CA159414/CA/NCI NIH HHS/United States ; U01 CA182370/CA/NCI NIH HHS/United States ; R01 CA164964/CA/NCI NIH HHS/United States
• Genre:
  Journal Article
• Place as Subject:
  Georgia ; Minnesota ; New York ; OSHA Region 2 ; OSHA Region 4 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  14 pdf pages
• Volume:
  4
• Issue:
  1
• NIOSHTIC Number:
  nn:20064562
• Contact Point Address:
  Jiyoung Ahn, Department of Population Health, New York University School of Medicine, New York, NY, USA
• Email:
  Jiyoung.Ahn@nyumc.org
• Federal Fiscal Year:
  2017
• Performing Organization:
  University of Minnesota Twin Cities
• Peer Reviewed:
  True
• Collection(s):
  National Institute for Occupational Safety and Health CDC Public Access
• Main Document Checksum:
  urn:sha-512:19501d527aee809d6611a84d2cd7f43c3f3ef65e9e330cb346b236f09442b5a6172c52eb05991703aa5a2c8da9b5ddb6d478335a0ff1b1a4b069a87ad30ed4f1
• Download URL:
  https://stacks.cdc.gov/view/cdc/43645/cdc_43645_DS1.pdf
• File Type:
  [PDF - 3.72 MB ]