Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration

Details

• Alternative Title:
  Virol J
• Personal Author:
  Stern, Daniel ; Olson, Victoria A. ; Smith, Scott K. ; Pietraszczyk, Marko ; Miller, Lilija ; Miethe, Peter ; Dorner, Brigitte G. ; Nitsche, Andreas
• Description:
  Background ; The rapid and reliable detection of infectious agents is one of the most challenging tasks in scenarios lacking well-equipped laboratory infrastructure, like diagnostics in rural areas of developing countries. Commercially available point-of-care diagnostic tests for emerging and rare diseases are particularly scarce. ; Results ; In this work we present a point-of-care test for the detection of Orthopoxviruses (OPV). The OPV ABICAP assay detects down to 1 × 104 plaque forming units/mL of OPV particles within 45 min. It can be applied to clinical material like skin crusts and detects all zoonotic OPV infecting humans, including Vaccinia, Cowpox, Monkeypox, and most importantly Variola virus. ; Conclusions ; Given the high sensitivity and the ease of handling, the novel assay could be highly useful for on-site diagnostics of suspected Monkeypox virus infections in areas lacking proper laboratory infrastructure as well as rapid on-site testing of suspected bioterrorism samples. ; Electronic supplementary material ; The online version of this article (doi:10.1186/s12985-016-0665-5) contains supplementary material, which is available to authorized users.
• Subjects:
  • Animals
  • Filtration
  • Humans
  • Immunoassay
  • Orthopoxvirus
  • Point-of-Care Systems
  • Poxviridae Infections
  • Sensitivity and Specificity
  • Time Factors
  • Virology
• Source:
  Virol J. 13.
• Pubmed ID:
  27938377
• Pubmed Central ID:
  PMC5148848
• Document Type:
  Journal Article
• Volume:
  13
• Download URL:
  https://stacks.cdc.gov/view/cdc/43642/cdc_43642_DS1.pdf
• File Type:
  [PDF - 1.78 MB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:7390f5f052355a0ee80ecaf4f6fb53013a0b2b0c3f0308d305fd5ad493efb6de