Detection of North American orthopoxviruses by real time-PCR

Gallardo-Romero, Nadia F.; Velasco-Villa, Andres; Weiss, Sonja L.; Emerson, Ginny L.; Carroll, Darin S.; Hughes, Christine M.; Li, Yu; Karem, Kevin L.; Damon, Inger K.; Olson, Victoria A.

Back to Previous Page

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

Detection of North American orthopoxviruses by real time-PCR

Published Date:

Jun 20 2011

Source:

Virol J. 2011; 8:313.

[PDF-1.82 MB]

Details:

Alternative Title:

Virol J

Personal Author:

Gallardo-Romero, Nadia F. ; Velasco-Villa, Andres ; Weiss, Sonja L. ; Emerson, Ginny L. ; Carroll, Darin S. ; ... More ▼

Description:

The prevalence of North American orthopoxviruses in nature is unknown and may be more difficult to ascertain due to wide spread use of vaccinia virus recombinant vaccines in the wild. A real time PCR assay was developed to allow for highly sensitive and specific detection of North American orthopoxvirus DNA in animal tissues and bodily fluids. This method is based on the amplification of a 156 bp sequence within a myristylated protein, highly conserved within the North American orthopoxviruses but distinct from orthologous genes present in other orthopoxviruses. The analytical sensitivity was 1.1 fg for Volepox virus DNA, 1.99 fg for Skunkpox virus DNA, and 6.4 fg for Raccoonpox virus DNA with a 95% confidence interval. Our assay did not cross-react with other orthopoxviruses or ten diverse representatives of the Chordopoxvirinae subfamily. This new assay showed more sensitivity than tissue culture tests, and was capable of differentiating North American orthopoxviruses from other members of Orthopoxvirus. Thus, our assay is a promising tool for highly sensitive and specific detection of North American orthopoxviruses in the United States and abroad.

Subject:

[+]

Document Type:

Journal Article

Place as Subject:

North America

Collection(s):

CDC Public Access

Main Document Checksum:

[+]

Supporting Files:

	license.txt	text/plain
	1743-422X-8-313-1.gif	image/gif
	1743-422X-8-313-1.jpg	image/jpeg
	1743-422X-8-313-2.gif	image/gif
	1743-422X-8-313-2.jpg	image/jpeg
	1743-422X-8-313-3.gif	image/gif
	1743-422X-8-313-3.jpg	image/jpeg
	1743-422X-8-313.nxml	text/plain

No Related Documents.

You May Also Like:

Human Infection with a Zoonotic Orthopoxvirus in the Country of Georgia

Personal Author:

Vora, Neil M.; Li, Yu; Geleishvili, Marika; Emerson, Ginny L.; Khmaladze, Ekaterine; Maghlakelidze, Giorgi; Navdarashvili, Archil; Zakhashvili, Khatuna; Kokhreidze, Maka; Endeladze, Marina; Mokverashvili, Gela; Satheshkumar, P.S.; Gallardo-Romero, Nadia; Goldsmith, Cynthia S.; Metcalfe, Maureen G.; Damon, Inger; Maes, Edmond F.; Reynolds, Mary G.; Morgan, Juliette; Carroll, Darin S.;

Published Date:

Mar 26 2015

Source:

N Engl J Med. 372(13):1223-1230.

Description:

During 2013, cutaneous lesions developed in two men in the country of Georgia after they were exposed to ill cows. The men had never received vaccination against smallpox. Tests of lesion material with the use of a quantitative real-time polymerase-c...

File Type:

[PDF - 1.41 MB]
Fatal Outbreak in Tonkean Macaques Caused by Possibly Novel Orthopoxvirus, Italy, January 20151

Personal Author:

Cardeti, Giusy; Gruber, Cesare Ernesto Maria; Eleni, Claudia; Carletti, Fabrizio; Castilletti, Concetta; Manna, Giuseppe; Rosone, Francesca; Giombini, Emanuela; Selleri, Marina; Lapa, Daniele; Puro, Vincenzo; Di Caro, Antonino; Lorenzetti, Raniero; Scicluna, Maria Teresa; Grifoni, Goffredo; Rizzoli, Annapaola; Tagliapietra, Valentina; De Marco, Lorenzo; Capobianchi, Maria Rosaria; Autorino, Gian Luca;

Published Date:

Dec 17 2017

Source:

Emerg Infect Dis. 23(12):1941-1949.

Description:

In January 2015, during a 3-week period, 12 captive Tonkean macacques at a sanctuary in Italy died. An orthopoxvirus infection was suspected because of negative-staining electron microscopy results. The diagnosis was confirmed by histology, virus iso...

File Type:

[PDF - 4.94 MB]
The Pox in the North American Backyard: Volepox Virus Pathogenesis in California Mice (Peromyscus californicus)

Personal Author:

Gallardo-Romero, Nadia F.; Drew, Clifton P.; Weiss, Sonja L.; Metcalfe, Maureen G.; Nakazawa, Yoshinori J.; Smith, Scott K.; Emerson, Ginny L.; Hutson, Christina L.; Salzer, Johanna S.; Bartlett, Jeanine H.; Olson, Victoria A.; Clemmons, Cody J.; Davidson, Whitni B.; Zaki, Sherif R.; Karem, Kevin L.; Damon, Inger K.; Carroll, Darin S.;

Published Date:

Aug 28 2012

Source:

PLoS One. 2012; 7(8).

Description:

Volepox virus (VPXV) was first isolated in 1985 from a hind foot scab of an otherwise healthy California vole (Microtus californicus). Subsequent surveys in San Mateo County, CA, revealed serological evidence suggesting that VPXV is endemic to this a...

File Type:

[PDF - 6.61 MB]
Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration

Personal Author:

Stern, Daniel; Olson, Victoria A.; Smith, Scott K.; Pietraszczyk, Marko; Miller, Lilija; Miethe, Peter; Dorner, Brigitte G.; Nitsche, Andreas;

Published Date:

Dec 09 2016

Source:

Virol J. 13.

Description:

BackgroundThe rapid and reliable detection of infectious agents is one of the most challenging tasks in scenarios lacking well-equipped laboratory infrastructure, like diagnostics in rural areas of developing countries. Commercially available point-o...

File Type:

[PDF - 1.78 MB]
Novel Orthopoxvirus and Lethal Disease in Cat, Italy

Personal Author:

Lanave, Gianvito; Dowgier, Giulia; Decaro, Nicola; Albanese, Francesco; Brogi, Elisa; Parisi, Antonio; Losurdo, Michele; Lavazza, Antonio; Martella, Vito; Buonavoglia, Canio; Elia, Gabriella;

Published Date:

Sep 2018

Source:

Emerg Infect Dis. 24(9):1665-1673.

Description:

We report detection and full-genome characterization of a novel orthopoxvirus (OPXV) responsible for a fatal infection in a cat. The virus induced skin lesions histologically characterized by leukocyte infiltration and eosinophilic cytoplasmic inclus...

File Type:

[PDF - 3.38 MB]