Evaluation of the Illumigene Malaria LAMP: A Robust Molecular Diagnostic Tool for Malaria Parasites

Lucchi, Naomi W.; Gaye, Marie; Diallo, Mammadou Alpha; Goldman, Ira F.; Ljolje, Dragan; Deme, Awa Bineta; Badiane, Aida; Ndiaye, Yaye Die; Barnwell, John W.; Udhayakumar, Venkatachalam; Ndiaye, Daouda

doi:10.1038/srep36808

i

Evaluation of the Illumigene Malaria LAMP: A Robust Molecular Diagnostic Tool for Malaria Parasites

Supporting Files Public Domain

Nov 09 2016
By Lucchi, Naomi W. ; Gaye, Marie ; Diallo, Mammadou Alpha ; ...

File Language:

English

Details

Alternative Title:

Sci Rep
Personal Author:

Lucchi, Naomi W. ; Gaye, Marie ; Diallo, Mammadou Alpha ; Goldman, Ira F. ; Ljolje, Dragan ; Deme, Awa Bineta ; Badiane, Aida ; Ndiaye, Yaye Die ; Barnwell, John W. ; Udhayakumar, Venkatachalam ; Ndiaye, Daouda
Description:

Isothermal nucleic acid amplification assays such as the loop mediated isothermal amplification (LAMP), are well suited for field use as they do not require thermal cyclers to amplify the DNA. To further facilitate the use of LAMP assays in remote settings, simpler sample preparation methods and lyophilized reagents are required. The performance of a commercial malaria LAMP assay (Illumigene Malaria LAMP) was evaluated using two sample preparation workflows (simple filtration prep (SFP)) and gravity-driven filtration prep (GFP)) and pre-dispensed lyophilized reagents. Laboratory and clinical samples were tested in a field laboratory in Senegal and the results independently confirmed in a reference laboratory in the U.S.A. The Illumigene Malaria LAMP assay was easily implemented in the clinical laboratory and gave similar results to a real-time PCR reference test with limits of detection of ≤2.0 parasites/μl depending on the sample preparation method used. This assay reliably detected Plasmodium sp. parasites in a simple low-tech format, providing a much needed alternative to the more complex molecular tests for malaria diagnosis.
Subjects:

DNA, Protozoan Humans Malaria Molecular Diagnostic Techniques Nucleic Acid Amplification Techniques Plasmodium Sensitivity And Specificity
Source:

Sci Rep. 2016; 6.
Pubmed ID:

27827432
Pubmed Central ID:

PMC5101795
Document Type:

Journal Article
Volume:

6
Collection(s):

CDC Public Access
Main Document Checksum:

urn:sha256:b979210e5325ab89ed20c0c939f1332e9d42856aa0fbba61dcb28a08c91687ec
Download URL:

https://stacks.cdc.gov/view/cdc/43058/cdc_43058_DS1.pdf
File Type:

[PDF - 613.13 KB ]

File Language:

English

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