Details:

Alternative Title:
PLoS One
Personal Author:
Kalb, Suzanne R. ; Garcia-Rodriguez, Consuelo ; Lou, Jianlong ; Baudys, Jakub ; Smith, Theresa J. ; ... More +
Kalb, Suzanne R. ; Garcia-Rodriguez, Consuelo ; Lou, Jianlong ; Baudys, Jakub ; Smith, Theresa J. ; Marks, James D. ; Smith, Leonard A. ; Pirkle, James L. ; Barr, John R. Less -
Description:
Botulinum neurotoxins (BoNTs) are extremely potent toxins that are capable of causing respiratory failure leading to long-term intensive care or death. The best treatment for botulism includes serotype-specific antitoxins, which are most effective when administered early in the course of the intoxication. Early confirmation of human exposure to any serotype of BoNT is an important public health goal. In previous work, we focused on developing Endopep-MS, a mass spectrometry-based endopeptidase method for detecting and differentiating the seven serotypes (BoNT/A-G) in buffer and BoNT/A, /B, /E, and /F (the four serotypes that commonly affect humans) in clinical samples. We have previously reported the success of antibody-capture to purify and concentrate BoNTs from complex matrices, such as clinical samples. However, to check for any one of the four serotypes of BoNT/A, /B, /E, or /F, each sample is split into 4 aliquots, and tested for the specific serotypes separately. The discovery of a unique monoclonal antibody that recognizes all four serotypes of BoNT/A, /B, /E and /F allows us to perform simultaneous detection of all of them. When applied in conjunction with the Endopep-MS assay, the detection limit for each serotype of BoNT with this multi-specific monoclonal antibody is similar to that obtained when using other serotype-specific antibodies.
Subject:
[+]
Amino Acid Sequence
Animals
Antibodies, Monoclonal
Antibody Specificity
Biochemistry
Botulinum Toxins
Botulinum Toxins, Type A
Cross Reactions
Endopeptidases
Female
Humans
Limit Of Detection
Mass Spectrometry
Mice
Molecular Sequence Data
Research Article

Source:
PLoS One. 5(8).
Document Type:
Journal Article
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:726751caaa7f8625de035e778125ff1b48e5d12a4931e3196931a50248c37876
File Type:

Supporting Files

• 	pone.0012237.g004.jpg	jpeg
  	pone.0012237.g005.gif	gif
  	pone.0012237.g005.jpg	jpeg
  	pone.0012237.g006.gif	gif
  	pone.0012237.g006.jpg	jpeg
  	pone.0012237.g007.gif	gif
  	pone.0012237.g007.jpg	jpeg
  	pone.0012237.nxml	txt
  	pone.0012237.t001.gif	gif
  	pone.0012237.t001.jpg	jpeg
  	license.txt	txt
  	pone.0012237.t002.gif	gif
  	pone.0012237.t002.jpg	jpeg
  	pone.0012237.g001.gif	gif
  	pone.0012237.g001.jpg	jpeg
  	pone.0012237.g002.gif	gif
  	pone.0012237.g002.jpg	jpeg
  	pone.0012237.g003.gif	gif
  	pone.0012237.g003.jpg	jpeg
  	pone.0012237.g004.gif	gif

More +