Survey of the Anti-Factor IX Immunoglobulin Profiles in Patients With Hemophilia B Using a Fluorescence-Based Immunoassay

Details

• Alternative Title:
  J Thromb Haemost
• Personal Author:
  Boylan, Brian ; Rice, Anne S. ; Neff, Anne T. ; Manco-Johnson, Marilyn J. ; Kempton, Christine L. ; Miller, Connie H.
• Description:
  Background ; Hemophilia B (HB) is an inherited bleeding disorder caused by the absence or dysfunction of coagulation factor IX (FIX). A subset of patients who have HB develop neutralizing alloantibodies (inhibitors) against FIX following infusion therapy. HB prevalence and the proportion of patients who develop inhibitors are much lower than that of hemophilia A (HA), which makes studies of inhibitors in patients with HB challenging due to the limited availability of samples. As a result, there is a knowledge gap regarding HB inhibitors. ; Objective ; Evaluate the largest group of inhibitor positive HB patients studied to date to assess the relationship between anti-FIX antibody profiles and inhibitor formation. ; Methods ; A fluorescence immunoassay (FLI) was used to detect anti-FIX antibodies in plasma samples from 37 patients with HB. ; Results ; Assessments of antibody profiles showed that anti-FIX IgG1-4, IgA, and IgE were detected significantly more often in patients with a positive Nijmegen-Bethesda Assay (NBA). All NBA-positive samples were positive for IgG4. Anti-FIX IgG4 demonstrated a strong correlation with the NBA, while correlations were significant, yet more moderate, for anti-FIX IgG1-2 and IgA. ; Conclusions ; The anti-FIX antibody profile in HB patients who develop inhibitors is diverse and correlates well with the NBA across immunoglobulin (sub)class, and anti-FIX IgG4 is particularly relevant to functional inhibition. The anti-FIX FLI may serve as a useful tool to confirm the presence of antibodies in patients who have low positive NBA results and to more clearly define, predict, and treat alloantibody formation against FIX.
• Subjects:
  • Adolescent
  • Adult
  • Aged
  • Antibodies, Neutralizing
  • Blood Coagulation
  • Case-Control Studies
  • Child
  • Child, Preschool
  • Cross-Sectional Studies
  • Factor IX
  More +
• Keywords:
  • Factor IX
  • Factor IX Deficiency
  • Hemophilia B
  • Inherited Blood Coagulation Disorders
  • Immunoassay
• Source:
  J Thromb Haemost. 14(10):1931-1940
• Pubmed ID:
  27501440
• Pubmed Central ID:
  PMC5083216
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHSUnited States/
• Volume:
  14
• Issue:
  10
• Download URL:
  https://stacks.cdc.gov/view/cdc/42303/cdc_42303_DS1.pdf
• File Type:
  [PDF - 689.09 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:587757a52697d3c9a8df25349587295d09addc5927afdcdd3e4dde43ce29e4d4