Digital imaging analysis to assess scar phenotype

Details

• Alternative Title:
  Wound Repair Regen
• Personal Author:
  Smith, Brian J. ; Nidey, Nichole ; Miller, Steven F. ; Moreno, Lina M. ; Baum, Christian L. ; Hamilton, Grant S. ; Wehby, George L. ; Dunnwald, Martine
• Description:
  In order to understand the link between the genetic background of patients and wound clinical outcomes, it is critical to have a reliable method to assess the phenotypic characteristics of healed wounds. In this study, we present a novel imaging method that provides reproducible, sensitive, and unbiased assessments of postsurgical scarring. We used this approach to investigate the possibility that genetic variants in orofacial clefting genes are associated with suboptimal healing. Red-green-blue digital images of postsurgical scars of 68 patients, following unilateral cleft lip repair, were captured using the 3dMD imaging system. Morphometric and colorimetric data of repaired regions of the philtrum and upper lip were acquired using ImageJ software, and the unaffected contralateral regions were used as patient-specific controls. Repeatability of the method was high with intraclass correlation coefficient score > 0.8. This method detected a very significant difference in all three colors, and for all patients, between the scarred and the contralateral unaffected philtrum (p ranging from 1.20(-05) to 1.95(-14) ). Physicians' clinical outcome ratings from the same images showed high interobserver variability (overall Pearson coefficient = 0.49) as well as low correlation with digital image analysis results. Finally, we identified genetic variants in TGFB3 and ARHGAP29 associated with suboptimal healing outcome.
• Subjects:
  • Adolescent
  • Adult
  • Child
  • Child, Preschool
  • Cicatrix
  • Cleft Lip
  • Color
  • Esthetics
  • Female
  • GTPase-Activating Proteins
  More +
• Keywords:
  • wound healing
  • cleft palate
  • genes
  • genotype-phenotype
  • Tgfb3
  • Arhgap29
  • scar
  • imaging
  • digital
• Source:
  Wound Repair Regen. 22(2):228-238
• Pubmed ID:
  24635173
• Pubmed Central ID:
  PMC4411947
• Document Type:
  Journal Article
• Funding:
  T32 DE014678/DE/NIDCR NIH HHSUnited States/ ; R37 DE008559/DE/NIDCR NIH HHSUnited States/ ; T90 DE023520/DE/NIDCR NIH HHSUnited States/ ; DE08559/DE/NIDCR NIH HHSUnited States/ ; T32 DEO14678-09/PHS HHSUnited States/ ; R03 AR055313/AR/NIAMS NIH HHSUnited States/ ; R01 DE008559/DE/NIDCR NIH HHSUnited States/ ; AR055313/AR/NIAMS NIH HHSUnited States/ ; R01 DD000295/DD/NCBDD CDC HHSUnited States/
• Volume:
  22
• Issue:
  2
• Download URL:
  https://stacks.cdc.gov/view/cdc/42038/cdc_42038_DS1.pdf
• File Type:
  [PDF - 850.16 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:943298534e65e912b130425deaed382cf01c76d48b0830b3df9e81daed4d8f01