Details:

Alternative Title:
P R Health Sci J
Personal Author:
Suárez, Erick ; González, Lorena ; Pérez-Mitchell, Carlos ; Ortiz, Ana P. ; Ramírez-Sola, Maricarmen ; ... More +
Suárez, Erick ; González, Lorena ; Pérez-Mitchell, Carlos ; Ortiz, Ana P. ; Ramírez-Sola, Maricarmen ; Acosta, Jaime ; Bernabe-Dones, Raúl D. ; González-Aquino, Carlos ; Montes-Rodríguez, Ingrid ; Cadilla, Carmen L. ; Less -
Description:
Objective The incidence of oral cavity and pharyngeal cancer in Puerto Rican men is higher than it is in the men of any other ethnic/racial group in the United States of America (US). The information regarding the effect of the human papilloma virus (HPV) in the gene-expression profile among patients with this cancer is limited in Hispanic community. We aim to describe the methodology for future studies to identify the molecular networks for determining overrepresented signaling and metabolic canonical pathways, based on the differential gene-expression profiles of HPV+ and HPV− samples from patients with oropharyngeal squamous cell carcinoma in Puerto Rico. Methods We analyzed the RNA expression of 5 tissue samples from subjects diagnosed with oropharyngeal squamous cell carcinoma, 2 HPV+ and 3 HPV−, using Affymetrix GeneChips. The relative difference between the average gene expressions of the HPV+ and HPV− samples was assessed, based on the fold change (log2-scale). Results Our analysis revealed 10 up regulated molecules (Mup1, LRP1, P14KA, ALYREF, and BHMT) and 5 down regulated ones (PSME4, KEAP1, ELK3, FAM186B, and PRELID1), at a cutoff of 1.5-fold change. Ingenuity Pathway Analysis showed the following biological functions to be affected in the HPV+ samples: cancer, hematological disease, and RNA post-transcriptional modification. QRT-PCR analysis confirmed only the differential regulation of ALYREF, KEAP1, and FAM186B genes. Conclusion The relevant methodological procedures described are sufficient to detect the most significant biological functions and pathways according to the HPV status in patients with oropharyngeal cancer in Puerto Rico.
Subject:
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Aged
Carcinoma, Squamous Cell
Female
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Head And Neck Neoplasms
Hispanic Americans
Humans
Male
Oligonucleotide Array Sequence Analysis
Oropharyngeal Neoplasms
Papillomaviridae
Papillomavirus Infections
Reverse Transcriptase Polymerase Chain Reaction
RNA Processing, Post-Transcriptional

Source:
P R Health Sci J. 35(1):3-8. ;
Pubmed ID:
26932277
Pubmed Central ID:
PMC4999560
Document Type:
Journal Article ;
Funding:
1U58DP000782-04/DP/NCCDPHP CDC HHS/United States ; 2U54MD007587/MD/NIMHD NIH HHS/United States ; 5R25CA094186-08/CA/NCI NIH HHS/United States ; G12 MD007600/MD/NIMHD NIH HHS/United States ; G12MD007600/MD/NIMHD NIH HHS/United States ; ... More +
1U58DP000782-04/DP/NCCDPHP CDC HHS/United States ; 2U54MD007587/MD/NIMHD NIH HHS/United States ; 5R25CA094186-08/CA/NCI NIH HHS/United States ; G12 MD007600/MD/NIMHD NIH HHS/United States ; G12MD007600/MD/NIMHD NIH HHS/United States ; R25 GM061838/GM/NIGMS NIH HHS/United States ; R25GM061838/GM/NIGMS NIH HHS/United States ; R25GM088023/GM/NIGMS NIH HHS/United States ; U54CA096297/CA/NCI NIH HHS/United States ; U54CA096300/CA/NCI NIH HHS/United States ; Less -
Place as Subject:
Puerto Rico ;
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:cdff2b03b5bbb044f8ca2613d0c8c77992749baa34c13a6ca9a36f01fe7eef76
File Type:

Supporting Files

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