Transgenic FingRs for Live Mapping of Synaptic Dynamics in Genetically-Defined Neurons

Details

• Alternative Title:
  Sci Rep
• Personal Author:
  Son, Jong-Hyun ; Keefe, Matthew D. ; Stevenson, Tamara J. ; Barrios, Joshua P. ; Anjewierden, Scott ; Newton, James B. ; Douglass, Adam D. ; Bonkowsky, Joshua L.
• Description:
  Tools for genetically-determined visualization of synaptic circuits and interactions are necessary to build connectomics of the vertebrate brain and to screen synaptic properties in neurological disease models. Here we develop a transgenic FingR (fibronectin intrabodies generated by mRNA display) technology for monitoring synapses in live zebrafish. We demonstrate FingR labeling of defined excitatory and inhibitory synapses, and show FingR applicability for dissecting synapse dynamics in normal and disease states. Using our system we show that chronic hypoxia, associated with neurological defects in preterm birth, affects dopaminergic neuron synapse number depending on the developmental timing of hypoxia.
• Subjects:
  Animals Animals, Genetically Modified Cell Tracking Fibronectins Fluorescent Antibody Technique Gene Expression Gene Order Genes, Reporter Genetic Vectors Hypoxia Immunohistochemistry Neurons Synapses Zebrafish

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• Source:
  Sci Rep. 2016; 6.
• Pubmed ID:
  26728131
• Pubmed Central ID:
  PMC4700522
• Document Type:
  Journal Article
• Funding:
  DP2 MH100008/DP/NCCDPHP CDC HHS/United States ; DP2 MH100008/MH/NIMH NIH HHS/United States ; R21 MH107039/MH/NIMH NIH HHS/United States
• Volume:
  6
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:52576026e97a781661b8985c06f42c2e246c95f31787fb0f8426b8aa089070d0
• Download URL:
  https://stacks.cdc.gov/view/cdc/41045/cdc_41045_DS1.pdf
• File Type:
  [PDF - 2.05 MB ]