Motor Behavior Mediated by Continuously Generated Dopaminergic Neurons in the Zebrafish Hypothalamus Recovers After Cell Ablation

Details

• Alternative Title:
  Curr Biol
• Personal Author:
  McPherson, Adam D. ; Barrios, Joshua P. ; Luks-Morgan, Sasha J. ; Manfredi, John P. ; Bonkowsky, Joshua L. ; Douglass, Adam D. ; Dorsky, Richard I.
• Description:
  Postembryonic neurogenesis has been observed in several regions of the vertebrate brain, including the dentate gyrus and rostral migratory stream in mammals, and is required for normal behavior [1-3]. Recently, the hypothalamus has also been shown to undergo continuous neurogenesis as a way to mediate energy balance [4-10]. As the hypothalamus regulates multiple functional outputs, it is likely that additional behaviors may be affected by postembryonic neurogenesis in this brain structure. Here, we have identified a progenitor population in the zebrafish hypothalamus that continuously generates neurons that express tyrosine hydroxylase 2 (th2). We develop and use novel transgenic tools to characterize the lineage of th2(+) cells and demonstrate that they are dopaminergic. Through genetic ablation and optogenetic activation, we then show that th2(+) neurons modulate the initiation of swimming behavior in zebrafish larvae. Finally, we find that the generation of new th2(+) neurons following ablation correlates with restoration of normal behavior. This work thus identifies for the first time a population of dopaminergic neurons that regulates motor behavior capable of functional recovery.
• Subjects:
  Animals Animals, Genetically Modified Behavior, Animal Dopamine Dopaminergic Neurons Hypothalamus Motor Activity Neurogenesis Zebrafish Zebrafish Proteins
• Source:
  Curr Biol. 26(2):263-269.
• Pubmed ID:
  26774784
• Pubmed Central ID:
  PMC4864152
• Document Type:
  Journal Article
• Funding:
  DP2 MH100008/MH/NIMH NIH HHS/United States ; T32 DC008553/DC/NIDCD NIH HHS/United States ; DP2 MH100008/DP/NCCDPHP CDC HHS/United States ; R01 NS082645/NS/NINDS NIH HHS/United States ; 5T32 HD07491/HD/NICHD NIH HHS/United States ; T32 HD007491/HD/NICHD NIH HHS/United States ; 5T32 NS076067/NS/NINDS NIH HHS/United States ; R01 NS053897/NS/NINDS NIH HHS/United States
• Volume:
  26
• Issue:
  2
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:8972ee014b4779ff5e29c31278a4bbf7c2568fa8d66ceba3956c5b425ac48ab9
• Download URL:
  https://stacks.cdc.gov/view/cdc/39521/cdc_39521_DS1.pdf
• File Type:
  [PDF - 2.14 MB ]