Details:

Alternative Title:
Curr Biol
Publisher's site:
http://dx.doi.org/10.1016/j.cub.2015.11.064
Personal Author:
McPherson, Adam D. ; Barrios, Joshua P. ; Luks-Morgan, Sasha J. ; Manfredi, John P. ; Bonkowsky, Joshua L. ; ... More +
McPherson, Adam D. ; Barrios, Joshua P. ; Luks-Morgan, Sasha J. ; Manfredi, John P. ; Bonkowsky, Joshua L. ; Douglass, Adam D. ; Dorsky, Richard I. ; Less -
Description:
Postembryonic neurogenesis has been observed in several regions of the vertebrate brain, including the dentate gyrus and rostral migratory stream in mammals, and is required for normal behavior [1-3]. Recently, the hypothalamus has also been shown to undergo continuous neurogenesis as a way to mediate energy balance [4-10]. As the hypothalamus regulates multiple functional outputs, it is likely that additional behaviors may be affected by postembryonic neurogenesis in this brain structure. Here, we have identified a progenitor population in the zebrafish hypothalamus that continuously generates neurons that express tyrosine hydroxylase 2 (th2). We develop and use novel transgenic tools to characterize the lineage of th2(+) cells and demonstrate that they are dopaminergic. Through genetic ablation and optogenetic activation, we then show that th2(+) neurons modulate the initiation of swimming behavior in zebrafish larvae. Finally, we find that the generation of new th2(+) neurons following ablation correlates with restoration of normal behavior. This work thus identifies for the first time a population of dopaminergic neurons that regulates motor behavior capable of functional recovery.
Subject:
[+]
Animals
Animals, Genetically Modified
Article
Behavior, Animal
Dopamine
Dopaminergic Neurons
Hypothalamus
Motor Activity
Neurogenesis
Zebrafish
Zebrafish Proteins

Source:
Curr Biol. 26(2):263-269. ;
Pubmed ID:
26774784
Pubmed Central ID:
PMC4864152
Document Type:
Journal Article ;
Funding:
DP2 MH100008/MH/NIMH NIH HHS/United States ; T32 DC008553/DC/NIDCD NIH HHS/United States ; DP2 MH100008/DP/NCCDPHP CDC HHS/United States ; R01 NS082645/NS/NINDS NIH HHS/United States ; 5T32 HD07491/HD/NICHD NIH HHS/United States ; ... More +
DP2 MH100008/MH/NIMH NIH HHS/United States ; T32 DC008553/DC/NIDCD NIH HHS/United States ; DP2 MH100008/DP/NCCDPHP CDC HHS/United States ; R01 NS082645/NS/NINDS NIH HHS/United States ; 5T32 HD07491/HD/NICHD NIH HHS/United States ; T32 HD007491/HD/NICHD NIH HHS/United States ; 5T32 NS076067/NS/NINDS NIH HHS/United States ; R01 NS053897/NS/NINDS NIH HHS/United States ; Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:8972ee014b4779ff5e29c31278a4bbf7c2568fa8d66ceba3956c5b425ac48ab9
File Type:

Supporting Files

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