Ancient human sialic acid variant restricts an emerging zoonotic malaria parasite

Dankwa, Selasi; Lim, Caeul; Bei, Amy K.; Jiang, Rays H. Y.; Abshire, James R.; Patel, Saurabh D.; Goldberg, Jonathan M.; Moreno, Yovany; Kono, Maya; Niles, Jacquin C.; Duraisingh, Manoj T.

doi:10.1038/ncomms11187

Back to Previous Page

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

Ancient human sialic acid variant restricts an emerging zoonotic malaria parasite

Published Date:

Apr 04 2016

Publisher's site:

http://dx.doi.org/10.1038/ncomms11187 New Site Icon

Source:

Nat Commun. 2016; 7.

[PDF-1.32 MB]

Details:

Personal Authors:

Dankwa, Selasi ; Lim, Caeul ; Bei, Amy K. ; Jiang, Rays H. Y. ; Abshire, James R. ; ... More ▼

Keywords:

[+]

Pubmed ID:

27041489

Pubmed Central ID:

PMC4822025

Description:

Plasmodium knowlesi is a zoonotic parasite transmitted from macaques causing malaria in humans in Southeast Asia. Plasmodium parasites bind to red blood cell (RBC) surface receptors, many of which are sialylated. While macaques synthesize the sialic acid variant N-glycolylneuraminic acid (Neu5Gc), humans cannot because of a mutation in the enzyme CMAH that converts N-acetylneuraminic acid (Neu5Ac) to Neu5Gc. Here we reconstitute CMAH in human RBCs for the reintroduction of Neu5Gc, which results in enhancement of P. knowlesi invasion. We show that two P. knowlesi invasion ligands, PkDBPβ and PkDBPγ, bind specifically to Neu5Gc-containing receptors. A human-adapted P. knowlesi line invades human RBCs independently of Neu5Gc, with duplication of the sialic acid-independent invasion ligand, PkDBPα and loss of PkDBPγ. Our results suggest that absence of Neu5Gc on human RBCs limits P. knowlesi invasion, but that parasites may evolve to invade human RBCs through the use of sialic acid-independent pathways.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

1DP2OD007124/OD/NIH HHS/United States
2-T32-AI007535-12/AI/NIAID NIH HHS/United States
5-K12-HD000850/HD/NICHD NIH HHS/United States
5-T32-GM08334/GM/NIGMS NIH HHS/United States
AI091787/AI/NIAID NIH HHS/United States
P50-GM098792/GM/NIGMS NIH HHS/United States
R36-CK000119-01/CK/NCEZID CDC HHS/United States
T32 GM008334/GM/NIGMS NIH HHS/United States

Supporting Files:

	ncomms11187-i1.jpg	image/jpeg
	ncomms11187-s1.pdf	application/pdf
	ncomms11187.nxml	application/octet-stream
	ncomms11187-f1.gif	image/gif
	ncomms11187-f1.jpg	image/jpeg
	ncomms11187-f2.gif	image/gif
	ncomms11187-f2.jpg	image/jpeg
	ncomms11187-f3.gif	image/gif
	ncomms11187-f3.jpg	image/jpeg
	ncomms11187-f4.gif	image/gif
	ncomms11187-f4.jpg	image/jpeg

No Related Documents.

You May Also Like:

Functional analysis of erythrocyte determinants of Plasmodium infection

Personal Author:

Bei, Amy K.; Duraisingh, Manoj T.;

Published:

Apr 19 2012

Source:

Int J Parasitol. 2012; 42(6):575-582.

Description:

The Plasmodium falciparum parasite is an obligate intracellular pathogen whose invasion and remodelling of the human erythrocyte results in the clinical manifestations of malarial disease. The functional analysis of erythrocyte determinants of invasi...

File Type:

[PDF - 828.84 KB]
An influenza A virus (H7N9) anti-neuraminidase monoclonal antibody with prophylactic and therapeutic activity in vivo

Personal Author:

Wilson, Jason R.; Guo, Zhu; Reber, Adrian; Kamal, Ram P.; Music, Nedzad; Gansebom, Shane; Bai, Yaohui; Levine, Min; Carney, Paul; Tzeng, Wen-Pin; Stevens, James; York, Ian A.;

Published:

Oct 03 2016

Source:

Antiviral Res. 135:48-55.

Description:

Zoonotic A(H7N9) avian influenza viruses emerged in China in 2013 and continue to be a threat to human public health, having infected over 800 individuals with a mortality rate approaching 40%. Treatment options for people infected with A(H7N9) inclu...

File Type:

[PDF - 1.06 MB]
New World Bats Harbor Diverse Influenza A Viruses

Personal Author:

Tong, Suxiang; Zhu, Xueyong; Li, Yan; Shi, Mang; Zhang, Jing; Bourgeois, Melissa; Yang, Hua; Chen, Xianfeng; Recuenco, Sergio; Gomez, Jorge; Chen, Li-Mei; Johnson, Adam; Tao, Ying; Dreyfus, Cyrille; Yu, Wenli; McBride, Ryan; Carney, Paul J.; Gilbert, Amy T.; Chang, Jessie; Guo, Zhu; Davis, Charles T.; Paulson, James C.; Stevens, James; Rupprecht, Charles E.; Holmes, Edward C.; Wilson, Ian A.; Donis, Ruben O.;

Published:

Oct 10 2013

Source:

PLoS Pathog. 2013; 9(10).

Description:

Aquatic birds harbor diverse influenza A viruses and are a major viral reservoir in nature. The recent discovery of influenza viruses of a new H17N10 subtype in Central American fruit bats suggests that other New World species may similarly carry div...

File Type:

[PDF - 2.21 MB]
A Plasmodium falciparum Homologue of Plasmodium vivax Reticulocyte Binding Protein (PvRBP1) Defines a Trypsin-resistant Erythrocyte Invasion Pathway

Personal Author:

Rayner, Julian C.; Vargas-Serrato, Esmeralda; Huber, Curtis S.; Galinski, Mary R.; Barnwell, John W.;

Published:

Dec 3 2001

Source:

J Exp Med. 194(11):1571-1582.

Description:

Invasion of erythrocytes by Plasmodium merozoites is an intricate process involving multiple receptor-ligand interactions. The glycophorins and an unknown trypsin sensitive factor are all erythrocyte receptors used during invasion by the major human ...

File Type:

[PDF - 215.46 KB]
Sowing the Seeds of a Pandemic? Mammalian Pathogenicity and Transmissibility of H1 Variant Influenza Viruses from the Swine Reservoir

Personal Author:

Pulit-Penaloza, Joanna A.; Belser, Jessica A.; Tumpey, Terrence M.; Maines, Taronna R.;

Published:

February 27 2019

Source:

Trop Med Infect Dis. 2019; 4(1)

Description:

Emergence of genetically and antigenically diverse strains of influenza to which the human population has no or limited immunity necessitates continuous risk assessments to determine the likelihood of these viruses acquiring adaptations that facilita...

File Type:

[PDF - 465.63 KB]