Siloxane nanoprobes for labeling and dual modality imaging of neural stem cells

Addington, Caroline P.; Cusick, Alex; Shankar, Rohini Vidya; Agarwal, Shubhangi; Stabenfeldt, Sarah E.; Kodibagkar, Vikram D.

doi:10.1007/s10439-015-1514-1

Back to Previous Page

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

Siloxane nanoprobes for labeling and dual modality imaging of neural stem cells

Published Date:

Nov 23 2015

Publisher's site:

http://dx.doi.org/10.1007/s10439-015-1514-1 New Site Icon

Source:

Ann Biomed Eng. 44(3):816-827.

[PDF-958.35 KB]

Details:

Alternative Title:

Ann Biomed Eng

Personal Author:

Addington, Caroline P. ; Cusick, Alex ; Shankar, Rohini Vidya ; Agarwal, Shubhangi ; Stabenfeldt, Sarah E. ; ... More ▼

Description:

Cell therapy represents a promising therapeutic for a myriad of medical conditions, including cancer, traumatic brain injury, and cardiovascular disease among others. A thorough understanding of the efficacy and cellular dynamics of these therapies necessitates the ability to non-invasively track cells in vivo. Magnetic resonance imaging (MRI) provides a platform to track cells as a non-invasive modality with superior resolution and soft tissue contrast. We recently reported a new nanoprobe platform for cell labeling and imaging using fluorophore doped siloxane core nanoemulsions as dual modality ((1)H MRI/Fluorescence), dual-functional (oximetry/detection) nanoprobes. Here, we successfully demonstrate the labeling, dual-modality imaging, and oximetry of neural progenitor/stem cells (NPSCs) in vitro using this platform. Labeling at a concentration of 10 μL/10(4) cells with a 40%v/v polydimethylsiloxane core nanoemulsion, doped with rhodamine, had minimal effect on viability, no effect on migration, proliferation and differentiation of NPSCs and allowed for unambiguous visualization of labeled NPSCs by (1)H MR and fluorescence and local pO2 reporting by labeled NPSCs. This new approach for cell labeling with a positive contrast (1)H MR probe has the potential to improve mechanistic knowledge of current therapies, and guide the design of future cell therapies due to its clinical translatability.

Subject:

[+]

Pubmed ID:

26597417

Pubmed Central ID:

PMC4792673

Document Type:

Journal Article

Funding:

DP2 HD084067/HD/NICHD NIH HHS/United States ; 1DP2HD084067/DP/NCCDPHP CDC HHS/United States ;

Collection(s):

CDC Public Access

Main Document Checksum:

[+]

Supporting Files:

	nihms740596f3.gif	image/gif
	nihms740596f3.jpg	image/jpeg
	nihms740596f4.gif	image/gif
	nihms740596f4.jpg	image/jpeg
	nihms740596f5.gif	image/gif
	nihms740596f5.jpg	image/jpeg
	nihms740596f6.gif	image/gif
	nihms740596f6.jpg	image/jpeg
	nihms740596f7.gif	image/gif
	nihms740596f7.jpg	image/jpeg
	NIHMS740596-supplement-10439_2015_1514_MOESM1_ESM.tif	image/tiff
	NIHMS740596-supplement-10439_2015_1514_MOESM2_ESM.tif	image/tiff
	NIHMS740596-supplement-10439_2015_1514_MOESM3_ESM.tif	image/tiff
	nihms740596.nxml	application/octet-stream
	nihms740596f1.gif	image/gif
	nihms740596f1.jpg	image/jpeg
	nihms740596f2.gif	image/gif
	nihms740596f2.jpg	image/jpeg

No Related Documents.

You May Also Like:

Real-Time Monitoring of ATP-Responsive Drug Release Using Mesoporous-Silica-Coated Multicolor Upconversion Nanoparticles

Personal Author:

Lai, Jinping; Shah, Birju P.; Zhang, Yixiao; Yang, Letao; Lee, Ki-Bum;

Published Date:

Apr 15 2015

Source:

ACS Nano. 9(5):5234-5245.

Description:

Stimuli-responsive drug delivery vehicles have garnered immense interest in recent years due to unparalleled progress made in material science and nanomedicine. However, the development of stimuli-responsive devices with integrated real-time monitori...

File Type:

[PDF - 1.45 MB]
Multimodal magnetic core-shell nanoparticles for effective stem cell differentiation and imaging**

Personal Author:

Shah, Birju; Yin, Perry T.; Lee, Ki-Bum;

Published Date:

May 06 2013

Source:

Angew Chem Int Ed Engl. 52(24):6190-6195.

File Type:

[PDF - 1.47 MB]
Probing stem cell behavior using nanoparticle-based approaches

Personal Author:

Patel, Sahishnu; Lee, Ki-Bum;

Published Date:

2015 Nov-Dec

Source:

Wiley Interdiscip Rev Nanomed Nanobiotechnol. 7(6):759-778.

Description:

Stem cells hold significant clinical potential to treat numerous debilitating diseases and injures that currently have no treatment plan. While several advances have been made in developing stem cell platforms and methods to induce their differentiat...

File Type:

[PDF - 1.74 MB]
Expansion Microscopy

Personal Author:

Chen, Fei; Tillberg, Paul W.; Boyden, Edward S.;

Published Date:

Jan 15 2015

Source:

Science. 347(6221):543-548.

Description:

In optical microscopy, fine structural details are resolved by using refraction to magnify images of a specimen. We discovered that by synthesizing a swellable polymer network within a specimen, it can be physically expanded, resulting in physical ma...

File Type:

[PDF - 1013.63 KB]
Photoacoustic and optical coherence tomography of epilepsy with high temporal and spatial resolution and dual optical contrasts Type of article: research article

Personal Author:

Tsytsarev, Vassiliy; Rao, Bin; Maslov, Konstantin I.; Li, Li; Wang, Lihong V.;

Published Date:

Apr 17 2013

Source:

J Neurosci Methods. 2013; 216(2):142-145

Description:

Epilepsy mapping with high spatial and temporal resolution has great significance for both fundamental research on epileptic neurons and the clinical management of epilepsy. In this communication, we demonstrate for the first time in vivo epilepsy ma...

File Type:

[PDF - 305.61 KB]