Siloxane nanoprobes for labeling and dual modality imaging of neural stem cells

Details

• Alternative Title:
  Ann Biomed Eng
• Personal Author:
  Addington, Caroline P. ; Cusick, Alex ; Shankar, Rohini Vidya ; Agarwal, Shubhangi ; Stabenfeldt, Sarah E. ; Kodibagkar, Vikram D.
• Description:
  Cell therapy represents a promising therapeutic for a myriad of medical conditions, including cancer, traumatic brain injury, and cardiovascular disease among others. A thorough understanding of the efficacy and cellular dynamics of these therapies necessitates the ability to non-invasively track cells in vivo. Magnetic resonance imaging (MRI) provides a platform to track cells as a non-invasive modality with superior resolution and soft tissue contrast. We recently reported a new nanoprobe platform for cell labeling and imaging using fluorophore doped siloxane core nanoemulsions as dual modality ((1)H MRI/Fluorescence), dual-functional (oximetry/detection) nanoprobes. Here, we successfully demonstrate the labeling, dual-modality imaging, and oximetry of neural progenitor/stem cells (NPSCs) in vitro using this platform. Labeling at a concentration of 10 μL/10(4) cells with a 40%v/v polydimethylsiloxane core nanoemulsion, doped with rhodamine, had minimal effect on viability, no effect on migration, proliferation and differentiation of NPSCs and allowed for unambiguous visualization of labeled NPSCs by (1)H MR and fluorescence and local pO2 reporting by labeled NPSCs. This new approach for cell labeling with a positive contrast (1)H MR probe has the potential to improve mechanistic knowledge of current therapies, and guide the design of future cell therapies due to its clinical translatability.
• Subjects:
  Animals Article Cell-labeling Cell Differentiation Cell Movement Cell Proliferation Cell Tracking Dual-modality Imaging Fluorescence Fluorescent Dyes Mice Molecular Imaging MR Oximetry Nanoemulsions Nanoparticles Neural Progenitor/stem Cells Neural Stem Cells Polymethyldisiloxane Rhodamine Siloxanes Staining And Labeling

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• Source:
  Ann Biomed Eng. 44(3):816-827.
• Pubmed ID:
  26597417
• Pubmed Central ID:
  PMC4792673
• Document Type:
  Journal Article
• Funding:
  DP2 HD084067/HD/NICHD NIH HHS/United States ; 1DP2HD084067/DP/NCCDPHP CDC HHS/United States
• Volume:
  44
• Issue:
  3
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:a0a01085d21ea551876e6e363e976df05294b784078732171abf62d930b04761
• Download URL:
  https://stacks.cdc.gov/view/cdc/40476/cdc_40476_DS1.pdf
• File Type:
  [PDF - 958.35 KB ]