Saccharomyces cerevisiae as a tool for mining, studying and engineering fungal polyketide synthases

Details

• Alternative Title:
  Fungal Genet Biol
• Personal Author:
  Bond, Carly ; Tang, Yi ; Li, Li
• Description:
  Small molecule secondary metabolites produced by organisms such as plants, bacteria, and fungi form a fascinating and important group of natural products, many of which have shown promise as medicines. Fungi in particular have been important sources of natural product polyketide pharmaceuticals. While the structural complexity of these polyketides makes them interesting and useful bioactive compounds, these same features also make them difficult and expensive to prepare and scale-up using synthetic methods. Currently, nearly all commercial polyketides are prepared through fermentation or semi-synthesis. However, elucidation and engineering of polyketide pathways in the native filamentous fungi hosts are often hampered due to a lack of established genetic tools and of understanding of the regulation of fungal secondary metabolisms. Saccharomyces cerevisiae has many advantages beneficial to the study and development of polyketide pathways from filamentous fungi due to its extensive genetic toolbox and well-studied metabolism. This review highlights the benefits S. cerevisiae provides as a tool for mining, studying, and engineering fungal polyketide synthases (PKSs), as well as notable insights this versatile tool has given us into the mechanisms and products of fungal PKSs.
• Subjects:
  Biological Products Homologous Recombination Metabolic Engineering Metabolic Networks And Pathways Polyketides Polyketide Synthases Saccharomyces Cerevisiae
• Source:
  Fungal Genet Biol. 89:52-61
• Pubmed ID:
  26850128
• Pubmed Central ID:
  PMC4789138
• Document Type:
  Journal Article
• Funding:
  DP1 GM106413/GM/NIGMS NIH HHSUnited States/ ; U01 GM110706/GM/NIGMS NIH HHSUnited States/ ; 1 U01 GM110706/GM/NIGMS NIH HHSUnited States/ ; 5 DP1 GM106413/DP/NCCDPHP CDC HHSUnited States/ ; 2 R01 GM085128/GM/NIGMS NIH HHSUnited States/ ; T32 GM067555/GM/NIGMS NIH HHSUnited States/ ; 2 T32 GM067555/GM/NIGMS NIH HHSUnited States/ ; R01 GM085128/GM/NIGMS NIH HHSUnited States/
• Volume:
  89
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:2087dfc124d599eaeff4af4e4bc566cb2f911cfb8d6e6bbe6b0c6f8007940de3
• Download URL:
  https://stacks.cdc.gov/view/cdc/40279/cdc_40279_DS1.pdf
• File Type:
  [PDF - 635.95 KB ]