Quantitative Multiscale Cell Imaging in Controlled 3D Microenvironments

Welf, Erik S.; Driscoll, Meghan K.; Dean, Kevin M.; Schäfer, Claudia; Chu, Jun; Davidson, Michael W.; Lin, Michael Z.; Danuser, Gaudenz; Fiolka, Reto

doi:10.1016/j.devcel.2016.01.022

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Quantitative Multiscale Cell Imaging in Controlled 3D Microenvironments

Published Date:

Feb 22 2016

Publisher's site:

http://dx.doi.org/10.1016/j.devcel.2016.01.022 New Site Icon

Source:

Dev Cell. 36(4):462-475.

[PDF-1.99 MB]

Details:

Personal Authors:

Welf, Erik S. ; Driscoll, Meghan K. ; Dean, Kevin M. ; Schäfer, Claudia ; Chu, Jun ; ... More ▼

Keywords:

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Pubmed ID:

26906741

Pubmed Central ID:

PMC4784259

Description:

The microenvironment determines cell behavior, but the underlying molecular mechanisms are poorly understood because quantitative studies of cell signaling and behavior have been challenging due to insufficient spatial and/or temporal resolution and limitations on microenvironmental control. Here we introduce microenvironmental selective plane illumination microscopy (meSPIM) for imaging and quantification of intracellular signaling and submicrometer cellular structures as well as large-scale cell morphological and environmental features. We demonstrate the utility of this approach by showing that the mechanical properties of the microenvironment regulate the transition of melanoma cells from actin-driven protrusion to blebbing, and we present tools to quantify how cells manipulate individual collagen fibers. We leverage the nearly isotropic resolution of meSPIM to quantify the local concentration of actin and phosphatidylinositol 3-kinase signaling on the surfaces of cells deep within 3D collagen matrices and track the many small membrane protrusions that appear in these more physiologically relevant environments.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

5DP1GM111003-02/DP/NCCDPHP CDC HHS/United States
DP1 GM111003/GM/NIGMS NIH HHS/United States
F32 GM116370/GM/NIGMS NIH HHS/United States
F32-GM116370/GM/NIGMS NIH HHS/United States

Main Document Checksum:

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