Details:

Alternative Title:
Dev Cell
Publisher's site:
http://dx.doi.org/10.1016/j.devcel.2016.01.022
Personal Author:
Welf, Erik S.; Driscoll, Meghan K.; Dean, Kevin M.; Schäfer, Claudia; Chu, Jun; ... More +
Welf, Erik S.; Driscoll, Meghan K.; Dean, Kevin M.; Schäfer, Claudia; Chu, Jun; Davidson, Michael W.; Lin, Michael Z.; Danuser, Gaudenz; Fiolka, Reto; Less -
Description:
The microenvironment determines cell behavior, but the underlying molecular mechanisms are poorly understood because quantitative studies of cell signaling and behavior have been challenging due to insufficient spatial and/or temporal resolution and limitations on microenvironmental control. Here we introduce microenvironmental selective plane illumination microscopy (meSPIM) for imaging and quantification of intracellular signaling and submicrometer cellular structures as well as large-scale cell morphological and environmental features. We demonstrate the utility of this approach by showing that the mechanical properties of the microenvironment regulate the transition of melanoma cells from actin-driven protrusion to blebbing, and we present tools to quantify how cells manipulate individual collagen fibers. We leverage the nearly isotropic resolution of meSPIM to quantify the local concentration of actin and phosphatidylinositol 3-kinase signaling on the surfaces of cells deep within 3D collagen matrices and track the many small membrane protrusions that appear in these more physiologically relevant environments.
Subject:
[+]
Actins
Article
Cell Culture Techniques
Cell Movement
Cells, Cultured
Collagen
Extracellular Matrix
Humans
Microscopy
Phosphatidylinositol 3-Kinases
Signal Transduction

Source:
Dev Cell. 36(4):462-475.;
Pubmed ID:
26906741
Pubmed Central ID:
PMC4784259
Document Type:
Journal Article;
Funding:
5DP1GM111003-02/DP/NCCDPHP CDC HHS/United States; DP1 GM111003/GM/NIGMS NIH HHS/United States; F32 GM116370/GM/NIGMS NIH HHS/United States; F32-GM116370/GM/NIGMS NIH HHS/United States;
5DP1GM111003-02/DP/NCCDPHP CDC HHS/United States; DP1 GM111003/GM/NIGMS NIH HHS/United States; F32 GM116370/GM/NIGMS NIH HHS/United States; F32-GM116370/GM/NIGMS NIH HHS/United States; Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:55e2c008c1f9f4aed082d52a4c330bf2828ee3cb86a910daf27a7fb47b90bdb3
File Type:

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