Stiffness-controlled three-dimensional extracellular matrices for high-resolution imaging of cell behavior

Details

• Alternative Title:
  Nat Protoc
• Personal Author:
  Fischer, Robert S ; Myers, Kenneth A ; Gardel, Margaret L ; Waterman, Clare M
• Description:
  Regulation of cell functions by the physical properties of the extracellular matrix (ECM) has emerged as a crucial contributor to development and disease. Two specific physical properties of the ECM, stiffness and dimensionality, each influence cell signaling and function. As these ECM physical properties are linked to other properties that also regulate cell behavior, e.g., integrin ligand density, parsing the specific contributions of ECM stiffness and dimensionality has proven difficult. Here we detail a simple protocol, which can be completed in 1-2 d, for combining three-dimensional (3D) ECM engagement with controlled underlying ECM stiffness. In these 'sandwich gels', cells are sandwiched between a 3D fibrillar ECM and an ECM-coupled polyacrylamide gel of defined compliance, allowing the study of the specific effects of ECM compliance on cell function in physiologically relevant 3D ECMs. This type of system enables high-resolution time-lapse imaging and is suitable for a wide range of cell types and molecular perturbations.
• Keywords:
  Acrylic Resins Cell Communication Cell Culture Techniques Cellular Microenvironment Extracellular Matrix Human Umbilical Vein Endothelial Cells Microscopy, Phase-Contrast
• Source:
  Nat Protoc. 2012; 7(11)
• Pubmed ID:
  23099487
• Pubmed Central ID:
  PMC3845971
• Document Type:
  Journal Article
• Funding:
  DP10D00354/DP/NCCDPHP CDC HHSUnited States/ ; K22 HL113069/HL/NHLBI NIH HHSUnited States/ ; ZIA HL005106-05/ImNIH/Intramural NIH HHSUnited States/ ; ZIA HL005106/ImNIH/Intramural NIH HHSUnited States/ ; Z99 HL999999/ImNIH/Intramural NIH HHSUnited States/
• Volume:
  7
• Issue:
  11
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:120a324f5543cc00ed0d33ea2d61c46ceac7f9ead3639130d81c4cd4abbd8baf
• Download URL:
  https://stacks.cdc.gov/view/cdc/33645/cdc_33645_DS1.pdf
• File Type:
  [PDF - 1.61 MB ]