Evaluation of the effect of valence state on cerium oxide nanoparticle toxicity following intratracheal instillation in rats

Details

• Alternative Title:
  Nanotoxicology
• Personal Author:
  Dunnick, Katherine M. ; Morris, Anna M. ; Badding, Melissa A. ; Barger, Mark ; Stefaniak, Aleksandr B. ; Sabolsky, Edward M. ; Leonard, Stephen S.
• Description:
  Cerium (Ce) is becoming a popular metal for use in electrochemical applications. When in the form of cerium oxide (CeO2), Ce can exist in both 3 + and 4 + valence states, acting as an ideal catalyst. Previous in vitro and in vivo evidence have demonstrated that CeO2 has either anti- or pro-oxidant properties, possibly due to the ability of the nanoparticles to transition between valence states. Therefore, we chose to chemically modify the nanoparticles to shift the valence state toward 3+. During the hydrothermal synthesis process, 10 mol% gadolinium (Gd) and 20 mol% Gd, were substituted into the lattice of the CeO2 nanoparticles forming a perfect solid solution with various A-site valence states. These two Gd-doped CeO2 nanoparticles were compared to pure CeO2 nanoparticles. Preliminary characteristics indicated that doping results in minimal size and zeta potential changes but alters valence state. Following characterization, male Sprague-Dawley rats were exposed to 0.5 or 1.0 mg/kg nanoparticles via a single intratracheal instillation. Animals were sacrificed and bronchoalveolar lavage fluid and various tissues were collected to determine the effect of valence state and oxygen vacancies on toxicity 1-, 7-, or 84-day post-exposure. Results indicate that damage, as measured by elevations in lactate dehydrogenase, occurred within 1-day post-exposure and was sustained 7-day post-exposure, but subsided to control levels 84-day post-exposure. Furthermore, no inflammatory signaling or lipid peroxidation occurred following exposure with any of the nanoparticles. Our results implicate that valence state has a minimal effect on CeO2 nanoparticle toxicity in vivo.
• Subjects:
  Animals Bronchoalveolar Lavage Fluid Cerium Dose-Response Relationship, Drug Gadolinium Instillation, Drug Lipid Peroxidation Lung Male Nanoparticles Oxygen Rats Rats, Sprague-Dawley Reactive Oxygen Species Signal Transduction

  More +
• Source:
  Nanotoxicology. 10(7):992-1000.
• Pubmed ID:
  26898289
• Pubmed Central ID:
  PMC4889510
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHS/United States
• Volume:
  10
• Issue:
  7
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:17d8e4688f435ee05c33d8212456502519c006d5e9f3586c55d55b9489a33e87
• Download URL:
  https://stacks.cdc.gov/view/cdc/39788/cdc_39788_DS1.pdf
• File Type:
  [PDF - 2.01 MB ]