Monitoring of Ebola Virus Makona Evolution through Establishment of Advanced Genomic Capability in Liberia

Kugelman, Jeffrey R.; Wiley, Michael R.; Mate, Suzanne; Ladner, Jason T.; Beitzel, Brett; Fakoli, Lawrence; Taweh, Fahn; Prieto, Karla; Diclaro, Joseph W.; Minogue, Timothy; Schoepp, Randal J.; Schaecher, Kurt E.; Pettitt, James; Bateman, Stacey; Fair, Joseph; Kuhn, Jens H.; Hensley, Lisa; Park, Daniel J.; Sabeti, Pardis C.; Sanchez-Lockhart, Mariano; Bolay, Fatorma K.; Palacios, Gustavo

doi:10.3201/eid2107.150522

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Monitoring of Ebola Virus Makona Evolution through Establishment of Advanced Genomic Capability in Liberia

Published Date:

Jul 2015

Publisher's site:

http://dx.doi.org/10.3201/eid2107.150522 New Site Icon

Source:

Emerg Infect Dis. 21(7):1135-1143.

[PDF - 2.47 MB]

Details:

Personal Authors:

Kugelman, Jeffrey R. ; Wiley, Michael R. ; Mate, Suzanne ; Ladner, Jason T. ; Beitzel, Brett ; ... More ▼

Corporate Authors:

on behalf of US Army Medical Research Institute of Infectious Diseases ; National Institutes of Health ; Integrated Research Facility–Frederick Ebola Response Team 2014–2015

Keywords:

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Pubmed ID:

26079255

Pubmed Central ID:

PMC4816332

Funding:

HHSN272200700016I/PHS HHS/United States

Document Type:

Journal Article

Collection(s):

Emerging Infectious Diseases

Description:

To support Liberia's response to the ongoing Ebola virus (EBOV) disease epidemic in Western Africa, we established in-country advanced genomic capabilities to monitor EBOV evolution. Twenty-five EBOV genomes were sequenced at the Liberian Institute for Biomedical Research, which provided an in-depth view of EBOV diversity in Liberia during September 2014-February 2015. These sequences were consistent with a single virus introduction to Liberia; however, shared ancestry with isolates from Mali indicated at least 1 additional instance of movement into or out of Liberia. The pace of change is generally consistent with previous estimates of mutation rate. We observed 23 nonsynonymous mutations and 1 nonsense mutation. Six of these changes are within known binding sites for sequence-based EBOV medical countermeasures; however, the diagnostic and therapeutic impact of EBOV evolution within Liberia appears to be low.

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