Monitoring of Ebola Virus Makona Evolution through Establishment of Advanced Genomic Capability in Liberia

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Kugelman, Jeffrey R. ; Wiley, Michael R. ; Mate, Suzanne ; Ladner, Jason T. ; Beitzel, Brett ; Fakoli, Lawrence ; Taweh, Fahn ; Prieto, Karla ; Diclaro, Joseph W. ; Minogue, Timothy ; Schoepp, Randal J. ; Schaecher, Kurt E. ; Pettitt, James ; Bateman, Stacey ; Fair, Joseph ; Kuhn, Jens H. ; Hensley, Lisa ; Park, Daniel J. ; Sabeti, Pardis C. ; Sanchez-Lockhart, Mariano ; Bolay, Fatorma K. ; Palacios, Gustavo
• Corporate Authors:
  on behalf of US Army Medical Research Institute of Infectious Diseases ; National Institutes of Health ; Integrated Research Facility–Frederick Ebola Response Team 2014–2015
• Description:
  To support Liberia's response to the ongoing Ebola virus (EBOV) disease epidemic in Western Africa, we established in-country advanced genomic capabilities to monitor EBOV evolution. Twenty-five EBOV genomes were sequenced at the Liberian Institute for Biomedical Research, which provided an in-depth view of EBOV diversity in Liberia during September 2014-February 2015. These sequences were consistent with a single virus introduction to Liberia; however, shared ancestry with isolates from Mali indicated at least 1 additional instance of movement into or out of Liberia. The pace of change is generally consistent with previous estimates of mutation rate. We observed 23 nonsynonymous mutations and 1 nonsense mutation. Six of these changes are within known binding sites for sequence-based EBOV medical countermeasures; however, the diagnostic and therapeutic impact of EBOV evolution within Liberia appears to be low.
• Subjects:
  Antiviral Agents DNA Mutational Analysis Drug Resistance, Viral Ebola Virus Ebolavirus Evolution, Molecular Expedited Filovirus Genes, Viral Genomics Hemorrhagic Fever, Ebola Humans Liberia Monitoring Of Ebola Virus Makona Evolution Through Establishment Of Advanced Genomic Capability In Liberia Negative-strand RNA Virus Research Viral Countermeasures Viral Hemorrhagic Fever Viruses

  More +
• Source:
  Emerg Infect Dis. 21(7):1135-1143
• Pubmed ID:
  26079255
• Pubmed Central ID:
  PMC4816332
• Document Type:
  Journal Article
• Funding:
  HHSN272200700016I/AO/NIAID NIH HHS/United States ; HHSN272200700016I/PHS HHS/United States
• Volume:
  21
• Issue:
  7
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:666e314f1dca4ddc4938982fd117182dd1c024ce9090383daf4a9a6595d80637
• Download URL:
  https://stacks.cdc.gov/view/cdc/39183/cdc_39183_DS1.pdf
• File Type:
  [PDF - 2.47 MB ]