Fluorescent and Bioluminescent Reporter Mouse-Adapted Ebola Viruses Maintain Pathogenicity and Can Be Visualized in Vivo

Details

• Alternative Title:
  J Infect Dis
• Personal Author:
  Davies, Katherine A. ; Welch, Stephen R. ; Jain, Shilpi ; Sorvillo, Teresa E. ; Coleman-McCray, JoAnn D. ; Montgomery, Joel M. ; Spiropoulou, Christina F. ; Albariño, César ; Spengler, Jessica R.
• Description:
  Ebola virus (EBOV) causes lethal disease in humans but not in mice. Here, we generated recombinant mouse-adapted (MA) EBOVs, including 1 based on the previously reported serially adapted strain (rMA-EBOV), along with single-reporter rMA-EBOVs expressing either fluorescent (ZsGreen1 [ZsG]) or bioluminescent (nano-luciferase [nLuc]) reporters, and dual-reporter rMA-EBOVs expressing both ZsG and nLuc. No detriment to viral growth in vitro was seen with inclusion of MA-associated mutations or reporter proteins. In CD-1 mice, infection with MA-EBOV, rMA-EBOV, and single-reporter rMA-EBOVs conferred 100% lethality; infection with dual-reporter rMA-EBOV resulted in 73% lethality. Bioluminescent signal from rMA-EBOV expressing nLuc was detected in vivo and ex vivo using the IVIS Spectrum CT. Fluorescent signal from rMA-EBOV expressing ZsG was detected in situ using handheld blue-light transillumination and ex vivo through epi-illumination with the IVIS Spectrum CT. These data support the use of reporter MA-EBOV for studies of Ebola virus in animal disease models.
• Subjects:
  Animals Ebola Vaccines Ebolavirus Hemorrhagic Fever, Ebola Humans Mice Mutation Virulence
• Keywords:
  Filovirus In Vivo Imaging Mouse-adapted Ebola Virus Reporter Viruses Viral Hemorrhagic Fever
• Source:
  J Infect Dis. 228(Suppl 7):S536-S547
• Pubmed ID:
  37145895
• Pubmed Central ID:
  PMC11014640
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  228
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:492130f8b9c9d1fd625aed754b5080f6b3e7dba1348be6290ed8f4c23fd3913b1d264bbbc71230613c5475e4194834f3229243fc8b9291bf03e32a320be6d5d7
• Download URL:
  https://stacks.cdc.gov/view/cdc/154197/cdc_154197_DS1.pdf
• File Type:
  [PDF - 2.21 MB ]