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Multi-scale photoacoustic tomography using reversibly switchable bacterial phytochrome as a near-infrared photochromic probe
  • Published Date:
    Nov 09 2015
  • Source:
    Nat Methods. 13(1):67-73.
Filetype[PDF - 1.52 MB]


Details:
  • Pubmed ID:
    26550774
  • Pubmed Central ID:
    PMC4697872
  • Description:
    Photoacoustic tomography (PAT) of genetically encoded probes allows for imaging of targeted biological processes deep in tissues with high spatial resolution; however, high background signals from blood can limit the achievable detection sensitivity. Here we describe a reversibly switchable nonfluorescent bacterial phytochrome for use in multiscale photoacoustic imaging, BphP1, with the most red-shifted absorption among genetically encoded probes. BphP1 binds a heme-derived biliverdin chromophore and is reversibly photoconvertible between red and near-infrared light-absorption states. We combined single-wavelength PAT with efficient BphP1 photoswitching, which enabled differential imaging with substantially decreased background signals, enhanced detection sensitivity, increased penetration depth and improved spatial resolution. We monitored tumor growth and metastasis with ∼ 100-μm resolution at depths approaching 10 mm using photoacoustic computed tomography, and we imaged individual cancer cells with a suboptical-diffraction resolution of ∼ 140 nm using photoacoustic microscopy. This technology is promising for biomedical studies at several scales.

  • Document Type:
  • Collection(s):
  • Funding:
    CA164468/CA/NCI NIH HHS/United States
    DP1 EB016986/DP/NCCDPHP CDC HHS/United States
    DP1 EB016986/EB/NIBIB NIH HHS/United States
    GM073913/GM/NIGMS NIH HHS/United States
    GM108579/GM/NIGMS NIH HHS/United States
    NS057105/NS/NINDS NIH HHS/United States
    P30 CA013330/CA/NCI NIH HHS/United States
    R01 CA164468/CA/NCI NIH HHS/United States
    R01 CA186567/CA/NCI NIH HHS/United States
    R01 CA186567/CA/NCI NIH HHS/United States
    R01 EB013571/EB/NIBIB NIH HHS/United States
    R01 EB016963/EB/NIBIB NIH HHS/United States
    R01 EB016963/EB/NIBIB NIH HHS/United States
    R01 GM073913/GM/NIGMS NIH HHS/United States
    R01 GM108579/GM/NIGMS NIH HHS/United States
    S10 RR026922/RR/NCRR NIH HHS/United States
    S10 RR026922/RR/NCRR NIH HHS/United States
    U01 NS090579/NS/NINDS NIH HHS/United States
    U01 NS090579/NS/NINDS NIH HHS/United States
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