Performance of rapid influenza diagnostic tests (QuickVue) for Influenza A and B Infection in India

Details

• Alternative Title:
  Indian J Med Microbiol
• Personal Author:
  Koul, PA ; Mir, H ; Bhat, MA ; Khan, UH ; Khan, MM ; Chadha, MS ; Lal, RB
• Description:
  Background
  
  Rapid point-of-care (POC) tests provide an economical alternative for rapid diagnosis and treatment of influenza, especially in public health emergency situations.
  
  Objectives
  
  To test the performance of a rapid influenza diagnostic test, QuickVue (Quidel) as a POC test against a real-time polymerase chain reaction (RT-PCR) assay for detection of influenza A and B in a developing country setting.
  
  Study Design
  
  In a prospective observational design, 600 patients with influenza-like illness (ILI) or with severe acute respiratory illness (SARI) who were referred to the Influenza Clinic of a tertiary care hospital in Srinagar, India from September 2012 to April 2013, were enrolled for diagnostic testing for influenza using QuickVue or RT-PCR. All influenza A-positive patients by RT-PCR were further subtyped using primers and probes for A/H1pdm09 and A/H3.
  
  Results
  
  Of the 600 patients, 186 tested positive for influenza A or B by RT-PCR (90 A/H1N1pdm09, 7 A/H3 and 89 influenza B), whereas only 43 tested positive for influenza (influenza A = 22 and influenza B = 21) by QuickVue. Thus, the sensitivity of the QuickVue was only 23% (95% confidence interval, CI: 17.3-29.8) and specificity was 100% (95% CI: 99.1-100) with a positive predictive value (PPV) of 100% (95% CI 91.8-100) and a negative predictive value (NPV) of 74.3% (95% CI: 70.5-77.9) as compared to RT-PCR.
  
  Conclusions
  
  The high specificity of QuickVue suggest that this POC test can be a useful tool for patient management or triaging during a public health crisis but a low sensitivity suggests that a negative test result need to be further tested using RT-PCR.
  
  
• Subjects:
  Adult Article Female Humans India Influenza Influenza, Human Influenza A Virus Influenza B Virus Male Point-of-Care Systems Rapid Tests Real-time PCR Real-Time Polymerase Chain Reaction Reproducibility Of Results Sensitivity Sensitivity And Specificity Young Adult

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• Source:
  Indian J Med Microbiol. 33(Suppl):26-31.
• Pubmed ID:
  25657152
• Pubmed Central ID:
  PMC4830807
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHS/United States
• Volume:
  33
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:528d39534f211b33cf0bd79d48dbfe22575749938f4a8751e92164106788f9d7
• Download URL:
  https://stacks.cdc.gov/view/cdc/38942/cdc_38942_DS1.pdf
• File Type:
  [PDF - 225.67 KB ]