Whole genome multilocus sequence typing as an epidemiologic tool for Yersinia pestis

Kingry, Luke C.; Rowe, Lori A.; Respicio-Kingry, Laurel B.; Beard, Charles B.; Schriefer, Martin E.; Petersen, Jeannine M.

doi:10.1016/j.diagmicrobio.2015.12.003

Back to Previous Page

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

Whole genome multilocus sequence typing as an epidemiologic tool for Yersinia pestis

Published Date:

Dec 12 2015

Publisher's site:

http://dx.doi.org/10.1016/j.diagmicrobio.2015.12.003 New Site Icon

Source:

Diagn Microbiol Infect Dis. 84(4):275-280.

[PDF-220.33 KB]

Details:

Personal Authors:

Kingry, Luke C. ; Rowe, Lori A. ; Respicio-Kingry, Laurel B. ; Beard, Charles B. ; Schriefer, Martin E. ; ... More ▼

Keywords:

[+]

Pubmed ID:

26778487

Pubmed Central ID:

PMC4829196

Description:

Human plague is a severe and often fatal zoonotic disease caused by Yersinia pestis. For public health investigations of human cases, nonintensive whole genome molecular typing tools, capable of defining epidemiologic relationships, are advantageous. Whole genome multilocus sequence typing (wgMLST) is a recently developed methodology that simplifies genomic analyses by transforming millions of base pairs of sequence into character data for each gene. We sequenced 13 US Y. pestis isolates with known epidemiologic relationships. Sequences were assembled de novo, and multilocus sequence typing alleles were assigned by comparison against 3979 open reading frames from the reference strain CO92. Allele-based cluster analysis accurately grouped the 13 isolates, as well as 9 publicly available Y. pestis isolates, by their epidemiologic relationships. Our findings indicate wgMLST is a simplified, sensitive, and scalable tool for epidemiologic analysis of Y. pestis strains.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

CC999999/Intramural CDC HHS/United States

Supporting Files:

	nihms775608.nxml	application/octet-stream
	nihms775608f1.gif	image/gif
	nihms775608f1.jpg	image/jpeg
	nihms775608f2.gif	image/gif
	nihms775608f2.jpg	image/jpeg

No Related Documents.

You May Also Like:

Investigation of and Response to 2 Plague Cases, Yosemite National Park, California, USA, 2015

Personal Author:

Danforth, Mary; Novak, Mark; Petersen, Jeannine; Mead, Paul; Kingry, Luke; Weinburke, Matthew; Buttke, Danielle; Hacker, Gregory; Tucker, James; Niemela, Michael; Jackson, Bryan; Padgett, Kerry; Liebman, Kelly; Vugia, Duc; Kramer, Vicki;

Published:

Dec 2016

Source:

Emerg Infect Dis. 22(12):2045-2053.

Description:

In August 2015, plague was diagnosed for 2 persons who had visited Yosemite National Park in California, USA. One case was septicemic and the other bubonic. Subsequent environmental investigation identified probable locations of exposure for each pat...

File Type:

[PDF - 704.50 KB]
Coxiella burnetii Genotyping

Personal Author:

Glazunova, Olga; Roux, Véronique; Freylikman, Olga; Sekeyova, Zuzana; Fournous, Ghislain; Tyczka, Judith; Tokarevich, Nikolai; Kovacova, Elena; Marrie, Thomas J.; Raoult, Didier;

Published:

Aug 2005

Source:

Emerg Infect Dis. 11(8):1211-1217.

Description:

Coxiella burnetii is a strict intracellular bacterium with potential as a bioterrorism agent. To characterize different isolates of C. burnetii at the molecular level, we performed multispacer sequence typing (MST). MST is based on intergenic region ...

File Type:

[PDF - 427.87 KB]
Human case of bubonic plague resulting from the bite of a wild Gunnison’s prairie dog during translocation from a plague-endemic area

Personal Author:

Melman, S. D.; Ettestad, P. E.; VinHatton, E. S.; Ragsdale, J. M.; Takacs, N.; Onischuk, L. M.; Leonard, P. M.; Master, S. S.; Lucero, V. S.; Kingry, L. C.; Petersen, J. M.;

Published:

Nov 06 2017

Source:

Zoonoses Public Health. 65(1):e254-e258.

Description:

Plague is a zoonotic disease (transmitted mainly by fleas and maintained in nature by rodents) that causes severe acute illness in humans. We present a human plague case who became infected by the bite of a wild Gunnison's prairie dog, and a good pra...

Public Access
Available on:

February 01, 2019
Canonical Insertion-Deletion Markers for Rapid DNA Typing of Francisella tularensis

Personal Author:

Larsson, Pär; Svensson, Kerstin; Karlsson, Linda; Guala, Dimitri; Granberg, Malin; Forsman, Mats; Johansson, Anders;

Published:

Nov 2007

Source:

Emerg Infect Dis. 13(11):1725-1732.

Description:

To develop effective and accurate typing of strains of Francisella tularensis, a potent human pathogen and a putative bioterrorist agent, we combined analysis of insertion-deletion (indel) markers with multiple-locus variable-number tandem repeat ana...

File Type:

[PDF - 459.94 KB]
Molecular Epidemiology of Glanders, Pakistan

Personal Author:

Hornstra, Heidie; Pearson, Talima; Georgia, Shalamar; Liguori, Andrew; Dale, Julia; Price, Erin; O’Neill, Matthew; DeShazer, David; Muhammad, Ghulam; Saqib, Muhammad; Naureen, Abeera; Keim, Paul;

Published:

Dec 2009

Source:

Emerg Infect Dis. 15(12):2036-2039.

Description:

We collected epidemiologic and molecular data from Burkholderia mallei isolates from equines in Punjab, Pakistan from 1999 through 2007. We show that recent outbreaks are genetically distinct from available whole genome sequences and that these genot...

File Type:

[PDF - 233.00 KB]