Whole genome multilocus sequence typing as an epidemiologic tool for Yersinia pestis

Kingry, Luke C.; Rowe, Lori A.; Respicio-Kingry, Laurel B.; Beard, Charles B.; Schriefer, Martin E.; Petersen, Jeannine M.

doi:10.1016/j.diagmicrobio.2015.12.003

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Whole genome multilocus sequence typing as an epidemiologic tool for Yersinia pestis

Published Date:

Dec 12 2015

Publisher's site:

http://dx.doi.org/10.1016/j.diagmicrobio.2015.12.003 New Site Icon

Source:

Diagn Microbiol Infect Dis. 84(4):275-280

Language:

English

[PDF-220.33 KB]

Details:

Personal Authors:

Kingry, Luke C. ; Rowe, Lori A. ; Respicio-Kingry, Laurel B. ; Beard, Charles B. ; Schriefer, Martin E. ; ... More ▼

Keywords:

[+]

Pubmed ID:

26778487

Pubmed Central ID:

PMC4829196

Description:

Human plague is a severe and often fatal zoonotic disease caused by Yersinia pestis. For public health investigations of human cases, nonintensive whole genome molecular typing tools, capable of defining epidemiologic relationships, are advantageous. Whole genome multilocus sequence typing (wgMLST) is a recently developed methodology that simplifies genomic analyses by transforming millions of base pairs of sequence into character data for each gene. We sequenced 13 US Y. pestis isolates with known epidemiologic relationships. Sequences were assembled de novo, and multilocus sequence typing alleles were assigned by comparison against 3979 open reading frames from the reference strain CO92. Allele-based cluster analysis accurately grouped the 13 isolates, as well as 9 publicly available Y. pestis isolates, by their epidemiologic relationships. Our findings indicate wgMLST is a simplified, sensitive, and scalable tool for epidemiologic analysis of Y. pestis strains.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

CC999999/Intramural CDC HHS/United States

Main Document Checksum:

[+]

Supporting Files:

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