Evaluation of Viremia Frequencies of a Novel Human Pegivirus by Using Bioinformatic Screening and PCR

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Bonsall, David ; Gregory, William F. ; Ip, Camilla L.C. ; Donfield, Sharyne ; Iles, James ; Ansari, M. Azim ; Piazza, Paolo ; Trebes, Amy ; Brown, Anthony ; Frater, John ; Pybus, Oliver G. ; Goulder, Phillip ; Klenerman, Paul ; Bowden, Rory ; Gomperts, Edward D. ; Barnes, Eleanor ; Kapoor, Amit ; Sharp, Colin P. ; Simmonds, Peter
• Description:
  Next-generation sequencing has critical applications in virus discovery, diagnostics, and environmental surveillance. We used metagenomic sequence libraries for retrospective screening of plasma samples for the recently discovered human hepegivirus 1 (HHpgV-1). From a cohort of 150 hepatitis C virus (HCV)-positive case-patients, we identified 2 persons with HHpgV-1 viremia and a high frequency of human pegivirus (HPgV) viremia (14%). Detection of HHpgV-1 and HPgV was concordant with parallel PCR-based screening using conserved primers matching groups 1 (HPgV) and 2 (HHPgV-1) nonstructural 3 region sequences. PCR identified 1 HHPgV-1-positive person with viremia from a group of 195 persons with hemophilia who had been exposed to nonvirally inactivated factor VII/IX; 18 (9%) were HPgV-positive. Relative to HCV and HPgV, active infections with HHpgV-1 were infrequently detected in blood, even in groups that had substantial parenteral exposure. Our findings are consistent with lower transmissibility or higher rates of virus clearance for HHpgV-1 than for other bloodborne human flaviviruses.
• Subjects:
  Bloodborne Pathogens Flaviviridae HCV Hemophilia Hepacivirus Hepegivirus HHpgV-1 Parenteral Pegivirus Persons Who Inject Drugs Research Sexually Transmitted Disease Transfusion Viremia Frequencies Of A Novel Human Pegivirus Evaluated By Using Bioinformatic Screening Viruses Virus Persistence

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• Source:
  Emerg Infect Dis. 22(4):671-678.
• Pubmed ID:
  26982117
• Pubmed Central ID:
  PMC4806942
• Document Type:
  Journal Article
• Volume:
  22
• Issue:
  4
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:6cf5898756aa547289c3f4755081402040e6d10ab16b673fe051123e3538687c
• Download URL:
  https://stacks.cdc.gov/view/cdc/38729/cdc_38729_DS1.pdf
• File Type:
  [PDF - 621.97 KB ]