Molecular Typing and Epidemiology of Human Listeriosis Cases, Denmark, 2002–20121

Jensen, Anne Kvistholm; Björkman, Jonas T.; Ethelberg, Steen; Kiil, Kristoffer; Kemp, Michael; Nielsen, Eva Møller

doi:10.3201/eid2204.150998

i

Molecular Typing and Epidemiology of Human Listeriosis Cases, Denmark, 2002–20121

Supporting Files Public Domain

Apr 2016
By Jensen, Anne Kvistholm ; Björkman, Jonas T. ; Ethelberg, Steen ; ...

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Jensen, Anne Kvistholm ; Björkman, Jonas T. ; Ethelberg, Steen ; Kiil, Kristoffer ; Kemp, Michael ; Nielsen, Eva Møller
Description:

Denmark has a high incidence of invasive listeriosis (0.9 cases/100,000 population in 2012). We analyzed patient data, clinical outcome, and trends in pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) of Listeria monocytogenes strains isolated in Denmark during 2002-2012. We performed 2-enzyme PFGE and serotyping on 559 isolates and MLST on 92 isolates and identified some correlation between molecular type and clinical outcome and patient characteristics. We found 178 different PFGE types, but isolates from 122 cases belonged to just 2 closely related PFGE types, clonal complex 8 and sequence type 8. These 2 types were the main cause of a peak in incidence of invasive listeriosis during 2005-2009, possibly representing an outbreak or the presence of a highly prevalent clone. However, current typing methods could not fully confirm these possibilities, highlighting the need for more refined discriminatory typing methods to identify outbreaks within frequently occurring L. monocytogenes PFGE types.
Subjects:

Bacteria Denmark Epidemiology Foodborne Diseases Humans Listeria Monocytogenes Listeriosis Molecular Epidemiology Molecular Typing And Epidemiology Of Human Listeriosis Cases, Denmark, 2002–2012 Multilocus Sequence Typing Pulsed-field Gel Electrophoresis Research Zoonoses
Source:

Emerg Infect Dis. 22(4):625-633.
Pubmed ID:

26982714
Pubmed Central ID:

PMC4806936
Document Type:

Journal Article
Volume:

22
Issue:

4
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:76acc2973b4104eec1b70daae563fb7bfe7cb2807a1c6f42832aa948a8eaaf6d
Download URL:

https://stacks.cdc.gov/view/cdc/38707/cdc_38707_DS1.pdf
File Type:

[PDF - 663.08 KB ]

ON THIS PAGE

Details Supporting Files

CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners.

As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.