Serological detection of West Nile virus in horses and chicken from Pantanal, Brazil

Details

• Alternative Title:
  Mem Inst Oswaldo Cruz
• Personal Author:
  Melandri, Vanessa ; Guimarães, Anthony Érico ; Komar, Nicholas ; Nogueira, Maurício L ; Mondini, Adriano ; Fernandez-Sesma, Ana ; Alencar, Jeronimo ; Bosch, Irene
• Description:
  In an effort to detect West Nile virus (WNV) in Brazil, we sampled serum from horses and chickens from the Pantanal region of the state of Mato Grosso and tested for flavivirus-reactive antibodies by blocking ELISA. The positive samples were further confirmed for serological evidence of WNV infection in three (8%) of the 38 horses and one (3.2%) of the 31 chickens using an 80% plaque-reduction neutralisation test (PRNT80). These results provide evidence of the circulation of WNV in chickens and horses in Pantanal.
• Subjects:
  Animals Antibodies, Viral Bird Diseases Brazil Chickens Enzyme-Linked Immunosorbent Assay Horse Diseases Horses Neutralization Tests Seroepidemiologic Studies West Nile Fever West Nile Virus

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• Keywords:
  B-ELISA PRNT WNV
• Source:
  Mem Inst Oswaldo Cruz. 107(8):1073-1075
• Pubmed ID:
  23295763
• Pubmed Central ID:
  PMC4782916
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  107
• Issue:
  8
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:3b76925da3dbdbbe4e721628364db19751c567853252007acdd44ba12fca37380a33696d97b910c3fd812cebe422af54766eaf85884730f6ff7d0270218cb202
• Download URL:
  https://stacks.cdc.gov/view/cdc/38383/cdc_38383_DS1.pdf
• File Type:
  [PDF - 428.83 KB ]