Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool

Hoenen, Thomas; Groseth, Allison; Rosenke, Kyle; Fischer, Robert J.; Hoenen, Andreas; Judson, Seth D.; Martellaro, Cynthia; Falzarano, Darryl; Marzi, Andrea; Squires, R. Burke; Wollenberg, Kurt R.; de Wit, Emmie; Prescott, Joseph; Safronetz, David; van Doremalen, Neeltje; Bushmaker, Trenton; Feldmann, Friederike; McNally, Kristin; Bolay, Fatorma K.; Fields, Barry; Sealy, Tara; Rayfield, Mark; Nichol, Stuart T.; Zoon, Kathryn C.; Massaquoi, Moses; Munster, Vincent J.; Feldmann, Heinz

doi:10.3201/eid2202.151796

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CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners. As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.

i

Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool

Feb 2016
By Hoenen, Thomas ; Groseth, Allison ; Rosenke, Kyle ; ...
http://dx.doi.org/10.3201/eid2202.151796
Source: Emerg Infect Dis. 22(2):331-334.

[PDF-500.67 KB]

English

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Details:

Alternative Title:

Emerg Infect Dis
Personal Author:

Hoenen, Thomas ; Groseth, Allison ; Rosenke, Kyle ; Fischer, Robert J. ; Hoenen, Andreas ; ... More +

Hoenen, Thomas ; Groseth, Allison ; Rosenke, Kyle ; Fischer, Robert J. ; Hoenen, Andreas ; Judson, Seth D. ; Martellaro, Cynthia ; Falzarano, Darryl ; Marzi, Andrea ; Squires, R. Burke ; Wollenberg, Kurt R. ; de Wit, Emmie ; Prescott, Joseph ; Safronetz, David ; van Doremalen, Neeltje ; Bushmaker, Trenton ; Feldmann, Friederike ; McNally, Kristin ; Bolay, Fatorma K. ; Fields, Barry ; Sealy, Tara ; Rayfield, Mark ; Nichol, Stuart T. ; Zoon, Kathryn C. ; Massaquoi, Moses ; Munster, Vincent J. ; Feldmann, Heinz Less -
Description:

Rapid sequencing of RNA/DNA from pathogen samples obtained during disease outbreaks provides critical scientific and public health information. However, challenges exist for exporting samples to laboratories or establishing conventional sequencers in remote outbreak regions. We successfully used a novel, pocket-sized nanopore sequencer at a field diagnostic laboratory in Liberia during the current Ebola virus outbreak.
Subjects:

[+]

Disease Outbreaks Dispatch Genome, Viral Hemorrhagic Fever, Ebola Humans Mutation Nanopores Sequence Analysis, DNA
Source:

Emerg Infect Dis. 22(2):331-334.
Pubmed ID:

26812583
Pubmed Central ID:

PMC4734547
Document Type:

Journal Article
Funding:

Intramural NIH HHS/United States
Place as Subject:

Africa, Western ; Liberia

Africa, Western ; Liberia Less -
Location:

Africa
Volume:

22
Issue:

2
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

[+]

urn:sha256:bd70d9dd33d9e39b715e9e8895cda2a32c5096d6f4abd4b6d076f7aaf67a3e0e
Download URL:

https://stacks.cdc.gov/view/cdc/38015/cdc_38015_DS1.pdf
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