Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool

Hoenen, Thomas; Groseth, Allison; Rosenke, Kyle; Fischer, Robert J.; Hoenen, Andreas; Judson, Seth D.; Martellaro, Cynthia; Falzarano, Darryl; Marzi, Andrea; Squires, R. Burke; Wollenberg, Kurt R.; de Wit, Emmie; Prescott, Joseph; Safronetz, David; van Doremalen, Neeltje; Bushmaker, Trenton; Feldmann, Friederike; McNally, Kristin; Bolay, Fatorma K.; Fields, Barry; Sealy, Tara; Rayfield, Mark; Nichol, Stuart T.; Zoon, Kathryn C.; Massaquoi, Moses; Munster, Vincent J.; Feldmann, Heinz

doi:10.3201/eid2202.151796

Back to Previous Page

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool

Published Date:

Feb 2016

Publisher's site:

http://dx.doi.org/10.3201/eid2202.151796 New Site Icon

Source:

Emerg Infect Dis. 22(2):331-334.

[PDF-500.67 KB]

Details:

Personal Authors:

Hoenen, Thomas ; Groseth, Allison ; Rosenke, Kyle ; Fischer, Robert J. ; Hoenen, Andreas ; ... More ▼

Keywords:

[+]

Pubmed ID:

26812583

Pubmed Central ID:

PMC4734547

Description:

Rapid sequencing of RNA/DNA from pathogen samples obtained during disease outbreaks provides critical scientific and public health information. However, challenges exist for exporting samples to laboratories or establishing conventional sequencers in remote outbreak regions. We successfully used a novel, pocket-sized nanopore sequencer at a field diagnostic laboratory in Liberia during the current Ebola virus outbreak.

Document Type:

Journal Article

Collection(s):

Emerging Infectious Diseases
CDC Public Access

Funding:

Intramural NIH HHS/United States

Place as Subject:

Africa, Western ; Liberia ;

Supporting Files:

	15-1796-F1.gif	image/gif
	15-1796-F1.jpg	image/jpeg
	15-1796-F2.gif	image/gif
	15-1796-F2.jpg	image/jpeg
	15-1796-Techapp-s1.pdf	application/pdf
	15-1796.nxml	application/octet-stream

No Related Documents.

You May Also Like:

Use of Unamplified RNA/cDNA–Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses

Personal Author:

Kilianski, Andy; Roth, Pierce A.; Liem, Alvin T.; Hill, Jessica M.; Willis, Kristen L.; Rossmaier, Rebecca D.; Marinich, Andrew V.; Maughan, Michele N.; Karavis, Mark A.; Kuhn, Jens H.; Honko, Anna N.; Rosenzweig, C. Nicole;

Published:

Aug 15 2016

Source:

Emerg Infect Dis. 22(8):1448-1451.

Description:

Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezu...

File Type:

[PDF - 825.52 KB]
Early Genomic Detection of Cosmopolitan Genotype of Dengue Virus Serotype 2, Angola, 2018

Personal Author:

Hill, Sarah C.; Neto de Vasconcelos, Jocelyne; Granja, Bernardo Gutierrez; Thézé, Julien; Jandondo, Domingos; Neto, Zoraima; Mirandela, Marinela; Sebastião, Cruz dos Santos; Cândido, Ana Luísa Micolo; Clemente, Carina; Pereira da Silva, Sara; de Oliveira, Túlio; Pybus, Oliver G.; Faria, Nuno R.; Afonso, Joana Morais;

Published:

Apr 2019

Source:

Emerg Infect Dis. 25(4):784-787

Description:

We used portable genome sequencing to investigate reported dengue virus transmission in Angola. Our results show that autochthonous transmission of dengue serotype 2 (cosmopolitan genotype) occurred in January 2018.

File Type:

[PDF - 1.66 MB]
Toward the Responsible Development and Commercialization of Sensor Nanotechnologies

Personal Author:

Fadel, Tarek R.; Farrell, Dorothy F.; Friedersdorf, Lisa E.; Griep, Mark H.; Hoover, Mark D.; Meador, Michael A.; Meyyappan, M.;

Published:

Feb 25 2016

Source:

ACS Sens. 1(3):207-216.

Description:

Nanotechnology-enabled sensors (or nanosensors) will play an important role in enabling the progression toward ubiquitous information systems as the Internet of Things (IoT) emerges. Nanosensors offer new, miniaturized solutions in physiochemical and...

File Type:

[PDF - 1.01 MB]
Next-Generation Sequencing Technologies and their Application to the Study and Control of Bacterial Infections

Personal Author:

Besser, John; Carleton, Heather A.; Gerner-Smidt, Peter; Lindsey, Rebecca L.; Trees, Eija;

Published:

Oct 23 2017

Source:

Clin Microbiol Infect. 24(4):335-341.

Description:

Background : With the decreasing cost and efficiency of next generation sequencing, the technology is rapidly introduced into clinical and public health laboratory practice. : Aims : In this review, the historical background and principles of first, ...

File Type:

[PDF - 210.87 KB]
Macrolide-Resistant Mycoplasma pneumoniae Infection, Japan, 2008–2015

Personal Author:

Tanaka, Takaaki; Oishi, Tomohiro; Miyata, Ippei; Wakabayashi, Shoko; Kono, Mina; Ono, Sahoko; Kato, Atsushi; Fukuda, Yoko; Saito, Aki; Kondo, Eisuke; Teranishi, Hideto; Tanaka, Yuhei; Wakabayashi, Tokio; Akaike, Hiroto; Ogita, Satoko; Ohno, Naoki; Nakano, Takashi; Terada, Kihei; Ouchi, Kazunobu;

Published:

Oct 2017

Source:

Emerg Infect Dis. 23(10):1703-1706.

Description:

We evaluated isolates obtained from children with Mycoplasma pneumoniae infection throughout Japan during 2008-2015. The highest prevalence of macrolide-resistant M. pneumoniae was 81.6% in 2012, followed by 59.3% in 2014 and 43.6% in 2015. The preva...

File Type:

[PDF - 1.03 MB]