Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool

Hoenen, Thomas; Groseth, Allison; Rosenke, Kyle; Fischer, Robert J.; Hoenen, Andreas; Judson, Seth D.; Martellaro, Cynthia; Falzarano, Darryl; Marzi, Andrea; Squires, R. Burke; Wollenberg, Kurt R.; de Wit, Emmie; Prescott, Joseph; Safronetz, David; van Doremalen, Neeltje; Bushmaker, Trenton; Feldmann, Friederike; McNally, Kristin; Bolay, Fatorma K.; Fields, Barry; Sealy, Tara; Rayfield, Mark; Nichol, Stuart T.; Zoon, Kathryn C.; Massaquoi, Moses; Munster, Vincent J.; Feldmann, Heinz

doi:10.3201/eid2202.151796

Back to Previous Page

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool

Published Date:

Feb 2016

Publisher's site:

http://dx.doi.org/10.3201/eid2202.151796 New Site Icon

Source:

Emerg Infect Dis. 22(2):331-334.

Language:

English

[PDF-500.67 KB]

Details:

Alternative Title:

Emerg Infect Dis

Personal Author:

Hoenen, Thomas ; Groseth, Allison ; Rosenke, Kyle ; Fischer, Robert J. ; Hoenen, Andreas ; ... More ▼

Description:

Rapid sequencing of RNA/DNA from pathogen samples obtained during disease outbreaks provides critical scientific and public health information. However, challenges exist for exporting samples to laboratories or establishing conventional sequencers in remote outbreak regions. We successfully used a novel, pocket-sized nanopore sequencer at a field diagnostic laboratory in Liberia during the current Ebola virus outbreak.

Subject:

[+]

Pubmed ID:

26812583

Pubmed Central ID:

PMC4734547

Document Type:

Journal Article

Funding:

Intramural NIH HHS/United States

Place as Subject:

Africa, Western ; Liberia ;

Collection(s):

Emerging Infectious Diseases
CDC Public Access

Main Document Checksum:

[+]

Supporting Files:

	15-1796-F1.gif	image/gif
	15-1796-F1.jpg	image/jpeg
	15-1796-F2.gif	image/gif
	15-1796-F2.jpg	image/jpeg
	15-1796-Techapp-s1.pdf	application/pdf
	15-1796.nxml	application/octet-stream

No Related Documents.

You May Also Like:

Use of Unamplified RNA/cDNA–Hybrid Nanopore Sequencing for Rapid Detection and Characterization of RNA Viruses

Personal Author:

Kilianski, Andy; Roth, Pierce A.; Liem, Alvin T.; Hill, Jessica M.; Willis, Kristen L.; Rossmaier, Rebecca D.; Marinich, Andrew V.; Maughan, Michele N.; Karavis, Mark A.; Kuhn, Jens H.; Honko, Anna N.; Rosenzweig, C. Nicole;

Published Date:

Aug 15 2016

Source:

Emerg Infect Dis. 22(8):1448-1451.

Description:

Nanopore sequencing, a novel genomics technology, has potential applications for routine biosurveillance, clinical diagnosis, and outbreak investigation of virus infections. Using rapid sequencing of unamplified RNA/cDNA hybrids, we identified Venezu...

File Type:

[PDF - 825.52 KB]
Metagenomic Approaches for Public Health Surveillance of Foodborne Infections: Opportunities and Challenges

Personal Author:

Carleton, Heather A.; Besser, John; Williams-Newkirk, Amanda J.; Huang, Andrew; Trees, Eija; Gerner-Smidt, Peter;

Published Date:

June 06 2019

Source:

Foodborne Pathog Dis. 2019; 16(7):474-479

Description:

Foodborne disease surveillance in the United States is at a critical point. Clinical and diagnostic laboratories are using culture-independent diagnostic tests (CIDTs) to identify the pathogen causing foodborne illness from patient specimens. CIDTs a...

File Type:

[PDF - 338.95 KB]
Early Genomic Detection of Cosmopolitan Genotype of Dengue Virus Serotype 2, Angola, 2018

Personal Author:

Hill, Sarah C.; Neto de Vasconcelos, Jocelyne; Granja, Bernardo Gutierrez; Thézé, Julien; Jandondo, Domingos; Neto, Zoraima; Mirandela, Marinela; Sebastião, Cruz dos Santos; Cândido, Ana Luísa Micolo; Clemente, Carina; Pereira da Silva, Sara; de Oliveira, Túlio; Pybus, Oliver G.; Faria, Nuno R.; Afonso, Joana Morais;

Published Date:

Apr 2019

Source:

Emerg Infect Dis. 25(4):784-787

Description:

We used portable genome sequencing to investigate reported dengue virus transmission in Angola. Our results show that autochthonous transmission of dengue serotype 2 (cosmopolitan genotype) occurred in January 2018.

File Type:

[PDF - 1.66 MB]
Toward the Responsible Development and Commercialization of Sensor Nanotechnologies

Personal Author:

Fadel, Tarek R.; Farrell, Dorothy F.; Friedersdorf, Lisa E.; Griep, Mark H.; Hoover, Mark D.; Meador, Michael A.; Meyyappan, M.;

Published Date:

Feb 25 2016

Source:

ACS Sens. 1(3):207-216.

Description:

Nanotechnology-enabled sensors (or nanosensors) will play an important role in enabling the progression toward ubiquitous information systems as the Internet of Things (IoT) emerges. Nanosensors offer new, miniaturized solutions in physiochemical and...

File Type:

[PDF - 1.01 MB]
Next Generation Sequencing of Infectious Pathogens

Personal Author:

Gwinn, Marta; MacCannell, Duncan; Armstrong, Gregory L.;

Published Date:

Mar 05 2019

Source:

JAMA. 321(9):893-894

Public Access
Available on:

March 05, 2020