Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Hoenen, Thomas ; Groseth, Allison ; Rosenke, Kyle ; Fischer, Robert J. ; Hoenen, Andreas ; Judson, Seth D. ; Martellaro, Cynthia ; Falzarano, Darryl ; Marzi, Andrea ; Squires, R. Burke ; Wollenberg, Kurt R. ; de Wit, Emmie ; Prescott, Joseph ; Safronetz, David ; van Doremalen, Neeltje ; Bushmaker, Trenton ; Feldmann, Friederike ; McNally, Kristin ; Bolay, Fatorma K. ; Fields, Barry ; Sealy, Tara ; Rayfield, Mark ; Nichol, Stuart T. ; Zoon, Kathryn C. ; Massaquoi, Moses ; Munster, Vincent J. ; Feldmann, Heinz
• Description:
  Rapid sequencing of RNA/DNA from pathogen samples obtained during disease outbreaks provides critical scientific and public health information. However, challenges exist for exporting samples to laboratories or establishing conventional sequencers in remote outbreak regions. We successfully used a novel, pocket-sized nanopore sequencer at a field diagnostic laboratory in Liberia during the current Ebola virus outbreak.
• Subjects:
  Disease Outbreaks Dispatch Genome, Viral Hemorrhagic Fever, Ebola Humans Mutation Nanopores Sequence Analysis, DNA
• Source:
  Emerg Infect Dis. 22(2):331-334.
• Pubmed ID:
  26812583
• Pubmed Central ID:
  PMC4734547
• Document Type:
  Journal Article
• Funding:
  Intramural NIH HHS/United States
• Place as Subject:
  Africa, Western ; Liberia
• Location:
  Africa
• Volume:
  22
• Issue:
  2
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:bd70d9dd33d9e39b715e9e8895cda2a32c5096d6f4abd4b6d076f7aaf67a3e0e
• Download URL:
  https://stacks.cdc.gov/view/cdc/38015/cdc_38015_DS1.pdf
• File Type:
  [PDF - 500.67 KB ]