Orthogonal gene knock out and activation with a catalytically active Cas9 nuclease

Dahlman, James E.; Abudayyeh, Omar O.; Joung, Julia; Gootenberg, Jonathan S.; Zhang, Feng; Konermann, Silvana

Back to Previous Page

Orthogonal gene knock out and activation with a catalytically active Cas9 nuclease

Published Date:

Nov 2015

Source:

Nat Biotechnol. 33(11):1159-1161.

[PDF-398.15 KB]

Details:

Alternative Title:

Nat Biotechnol

Personal Author:

Dahlman, James E. ; Abudayyeh, Omar O. ; Joung, Julia ; Gootenberg, Jonathan S. ; Zhang, Feng ; ... More ▼

Description:

We have developed a CRISPR-based method that uses catalytically active Cas9 and distinct single guide (sgRNA) constructs to knock out and activate different genes in the same cell. These sgRNAs, with 14- to 15-bp target sequences and MS2 binding loops, can activate gene expression using an active Streptococcus pyogenes Cas9 nuclease, without inducing double-stranded breaks. We use these 'dead RNAs' to perform orthogonal gene knockout and transcriptional activation in human cells.

Subject:

[+]

Pubmed ID:

26436575

Pubmed Central ID:

PMC4747789

Document Type:

Journal Article

Funding:

1DP1-MH100706/DP/NCCDPHP CDC HHS/United States ; DP1 MH100706/MH/NIMH NIH HHS/United States ;

Collection(s):

CDC Public Access

Main Document Checksum:

[+]

File Type:

Supporting Files:

	NIHMS726993-supplement-supplement_1.pdf	application/pdf
	nihms726993.nxml	application/octet-stream
	nihms726993f1.gif	image/gif
	nihms726993f1.jpg	image/jpeg
	nihms726993f2.gif	image/gif
	nihms726993f2.jpg	image/jpeg

No Related Documents.

Rationally engineered Cas9 nucleases with improved specificity

[PDF - 1.18 MB]

Personal Author:

Slaymaker, Ian M. ; Gao, Linyi ; ...

Published Date:

Dec 01 2015

Source:

Science. 351(6268):84-88.

Description:

The RNA-guided endonuclease Cas9 is a versatile genome-editing tool with a broad range of applications from therapeutics to functional annotation of g...

File Type:

[PDF - 1.18 MB]

File Type:

[PDF - 1.18 MB]
Cpf1 is a single RNA-guided endonuclease of a Class 2 CRISPR-Cas system

[PDF - 1.79 MB]

Personal Author:

Zetsche, Bernd ; Gootenberg, Jonathan S. ; ...

Published Date:

Sep 25 2015

Source:

Cell. 163(3):759-771.

Description:

The microbial adaptive immune system CRISPR mediates defense against foreign genetic elements through two classes of RNA-guided nuclease effectors. Cl...

File Type:

[PDF - 1.79 MB]

File Type:

[PDF - 1.79 MB]
CRISPR RNA-guided activation of endogenous human genes

[PDF - 206.72 KB]

Personal Author:

Maeder, Morgan L ; Linder, Samantha J ; ...

Published Date:

Jul 25 2013

Source:

Nat Methods. 10(10):977-979.

Description:

Short guide RNAs (gRNAs) can direct catalytically inactive CRISPR-associated 9 nuclease (dCas9) to repress endogenous genes in bacteria and human cell...

File Type:

[PDF - 206.72 KB]

File Type:

[PDF - 206.72 KB]
High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

[PDF - 337.75 KB]

Personal Author:

Fu, Yanfang ; Foden, Jennifer A. ; ...

Published Date:

Jun 23 2013

Source:

Nat Biotechnol. 31(9):822-826.

Description:

Clustered, regularly interspaced, short palindromic repeat (CRISPR) RNA-guided nucleases (RGNs) have rapidly emerged as a facile and efficient platfor...

File Type:

[PDF - 337.75 KB]

File Type:

[PDF - 337.75 KB]
AUTS2 confers gene activation to Polycomb group proteins in the CNS

[PDF - 4.27 MB]

Personal Author:

Gao, Zhonghua ; Lee, Pedro ; ...

Published Date:

Dec 18 2014

Source:

Nature. 516(7531):349-354.

Description:

Naturally occurring variations of Polycomb repressive complex 1 (PRC1) comprise a core assembly of Polycomb group proteins and additional factors that...

File Type:

[PDF - 4.27 MB]

File Type:

[PDF - 4.27 MB]

Orthogonal gene knock out and activation with a catalytically active Cas9 nuclease

Orthogonal gene knock out and activation with a catalytically active Cas9 nuclease

You May Also Like:

Have Questions?

CDC INFORMATION

CONNECT WITH CDC