Orthogonal gene knock out and activation with a catalytically active Cas9 nuclease
-
Published Date:Nov 2015
-
Source:Nat Biotechnol. 33(11):1159-1161.
Details:
-
Personal Authors:
-
Keywords:
-
Pubmed ID:26436575
-
Pubmed Central ID:PMC4747789
-
Description:We have developed a CRISPR-based method that uses catalytically active Cas9 and distinct single guide (sgRNA) constructs to knock out and activate different genes in the same cell. These sgRNAs, with 14- to 15-bp target sequences and MS2 binding loops, can activate gene expression using an active Streptococcus pyogenes Cas9 nuclease, without inducing double-stranded breaks. We use these 'dead RNAs' to perform orthogonal gene knockout and transcriptional activation in human cells.
-
Document Type:
-
Collection(s):
-
Funding:1DP1-MH100706/DP/NCCDPHP CDC HHS/United States
DP1 MH100706/MH/NIMH NIH HHS/United States
-
Supporting Files:
application/pdf application/octet-stream image/gif image/jpeg image/gif image/jpeg
No Related Documents.
You May Also Like: