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Orthogonal gene knock out and activation with a catalytically active Cas9 nuclease
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11 2015
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Source: Nat Biotechnol. 33(11):1159-1161
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Alternative Title:Nat Biotechnol
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Description:We have developed a CRISPR-based method that uses catalytically active Cas9 and distinct single guide (sgRNA) constructs to knock out and activate different genes in the same cell. These sgRNAs, with 14- to 15-bp target sequences and MS2 binding loops, can activate gene expression using an active Streptococcus pyogenes Cas9 nuclease, without inducing double-stranded breaks. We use these 'dead RNAs' to perform orthogonal gene knockout and transcriptional activation in human cells.
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Pubmed ID:26436575
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Pubmed Central ID:PMC4747789
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