Whole-brain activity mapping onto a zebrafish brain atlas

Details

• Alternative Title:
  Nat Methods
• Personal Author:
  Randlett, Owen ; Wee, Caroline L. ; Naumann, Eva A. ; Nnaemeka, Onyeka ; Schoppik, David ; Fitzgerald, James E. ; Portugues, Ruben ; Lacoste, Alix M.B. ; Riegler, Clemens ; Engert, Florian ; Schier, Alexander F.
• Description:
  In order to localize the neural circuits involved in generating behaviors, it is necessary to assign activity onto anatomical maps of the nervous system. Using brain registration across hundreds of larval zebrafish, we have built an expandable open-source atlas containing molecular labels and definitions of anatomical regions, the Z-Brain. Using this platform and immunohistochemical detection of phosphorylated extracellular signal–regulated kinase (ERK) as a readout of neural activity, we have developed a system to create and contextualize whole-brain maps of stimulus- and behavior-dependent neural activity. This mitogen-activated protein kinase (MAP)-mapping assay is technically simple, and data analysis is completely automated. Because MAP-mapping is performed on freely swimming fish, it is applicable to studies of nearly any stimulus or behavior. Here we demonstrate our high-throughput approach using pharmacological, visual and noxious stimuli, as well as hunting and feeding. The resultant maps outline hundreds of areas associated with behaviors.
• Subjects:
  Algorithms Animals Automation Behavior, Animal Brain Brain Mapping Calcium Extracellular Signal-Regulated MAP Kinases Image Processing, Computer-Assisted Immunohistochemistry Microscopy, Confocal Neurites Neurons Phosphorylation Principal Component Analysis Reproducibility Of Results Software Swimming Zebrafish

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• Source:
  Nat Methods. 12(11):1039-1046
• Pubmed ID:
  26778924
• Pubmed Central ID:
  PMC4710481
• Document Type:
  Journal Article
• Funding:
  DP1 NS082121/NS/NINDS NIH HHSUnited States/ ; R24 NS086601/NS/NINDS NIH HHSUnited States/ ; T32 HL007901/HL/NHLBI NIH HHSUnited States/ ; U01 MH105960/MH/NIMH NIH HHSUnited States/ ; DP1 NS082121-02/DP/NCCDPHP CDC HHSUnited States/ ; R01 HL109525/HL/NHLBI NIH HHSUnited States/ ; R00 DC012775/DC/NIDCD NIH HHSUnited States/ ; U01 NS090449/NS/NINDS NIH HHSUnited States/
• Volume:
  12
• Issue:
  11
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:15752ed027b80a3f21c1ad661532b02f0b38431c2ebb73fcb1ec0b51b8d25c07
• Download URL:
  https://stacks.cdc.gov/view/cdc/37799/cdc_37799_DS1.pdf
• File Type:
  [PDF - 2.52 MB ]