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Site-specific labeling of proteins for electron microscopy
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Sep 25 2015
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Source: J Struct Biol. 192(2):151-158
[PDF-1.03 MB]
Details:
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Alternative Title:J Struct Biol
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Personal Author:
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Description:Electron microscopy is commonly employed to determine the subunit organization of large macromolecular assemblies. However, the field lacks a robust molecular labeling methodology for unambiguous identification of constituent subunits. We present a strategy that exploits the unique properties of an unnatural amino acid in order to enable site-specific attachment of a single, readily identifiable protein label at any solvent-exposed position on the macromolecular surface. Using this method, we show clear labeling of a subunit within the 26S proteasome lid subcomplex that has not been amenable to labeling by traditional approaches.
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Pubmed ID:26409249
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Pubmed Central ID:PMC4633364
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Volume:192
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Issue:2
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