Human Heat shock protein 40 (Hsp40/DnaJB1) promotes influenza A virus replication by assisting nuclear import of viral ribonucleoproteins

Batra, Jyoti; Tripathi, Shashank; Kumar, Amrita; Katz, Jacqueline M.; Cox, Nancy J.; Lal, Renu B.; Sambhara, Suryaprakash; Lal, Sunil K.

doi:10.1038/srep19063

i

Human Heat shock protein 40 (Hsp40/DnaJB1) promotes influenza A virus replication by assisting nuclear import of viral ribonucleoproteins

Supporting Files Public Domain

Jan 11 2016
By Batra, Jyoti ; Tripathi, Shashank ; Kumar, Amrita ; ...

File Language:

English

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Alternative Title:

Sci Rep
Personal Author:

Batra, Jyoti ; Tripathi, Shashank ; Kumar, Amrita ; Katz, Jacqueline M. ; Cox, Nancy J. ; Lal, Renu B. ; Sambhara, Suryaprakash ; Lal, Sunil K.
Description:

A unique feature of influenza A virus (IAV) life cycle is replication of the viral genome in the host cell nucleus. The nuclear import of IAV genome is an indispensable step in establishing virus infection. IAV nucleoprotein (NP) is known to mediate the nuclear import of viral genome via its nuclear localization signals. Here, we demonstrate that cellular heat shock protein 40 (Hsp40/DnaJB1) facilitates the nuclear import of incoming IAV viral ribonucleoproteins (vRNPs) and is important for efficient IAV replication. Hsp40 was found to interact with NP component of IAV RNPs during early stages of infection. This interaction is mediated by the J domain of Hsp40 and N-terminal region of NP. Drug or RNAi mediated inhibition of Hsp40 resulted in reduced nuclear import of IAV RNPs, diminished viral polymerase function and attenuates overall viral replication. Hsp40 was also found to be required for efficient association between NP and importin alpha, which is crucial for IAV RNP nuclear translocation. These studies demonstrate an important role for cellular chaperone Hsp40/DnaJB1 in influenza A virus life cycle by assisting nuclear trafficking of viral ribonucleoproteins.
Subjects:

Benzhydryl Compounds Cell Line Cell Nucleus Gene Knockdown Techniques Host-Pathogen Interactions HSP40 Heat-Shock Proteins Humans Influenza, Human Influenza A Virus Models, Biological Protein Binding Protein Interaction Domains And Motifs Protein Transport Pyrrolidinones Ribonucleoproteins RNA, Small Interfering Viral Proteins Virus Replication
Source:

Sci Rep. 2016; 6.
Pubmed ID:

26750153
Pubmed Central ID:

PMC4707480
Document Type:

Journal Article
Volume:

6
Collection(s):

CDC Public Access
Main Document Checksum:

urn:sha256:97fd7827533859219e3c796f8c574db0f61d0b663364134f186b71071d15bdb7
Download URL:

https://stacks.cdc.gov/view/cdc/37752/cdc_37752_DS1.pdf
File Type:

[PDF - 1.82 MB ]

File Language:

English

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