Interactions of SARS Coronavirus Nucleocapsid Protein with the host cell proteasome subunit p42

Details

• Alternative Title:
  Virol J
• Personal Author:
  Wang, Qin ; Li, Chuan ; Zhang, Quanfu ; Wang, Tao ; Li, Jiandong ; Guan, Wuxiang ; Yu, Jianshi ; Liang, Mifang ; Li, Dexin
• Description:
  Background
  
  Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spreads rapidly and has a high case-mortality rate. The nucleocapsid protein (NP) of SARS-CoV may be critical for pathogenicity. This study sought to discover the host proteins that interact with SARS-CoV NP.
  
  Results
  
  Using surface plasmon resonance biomolecular interaction analysis (SPR/BIA) and matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry, we found that only the proteasome subunit p42 from human fetal lung diploid fibroblast (2BS) cells bound to SARS-CoV NP. This interaction was confirmed by the glutathione S-transferase (GST) fusion protein pulldown technique. The co-localization signal of SARS-CoV NP and proteasome subunit p42 in 2BS cells was detected using indirect immunofluorescence and confocal microscopy. p42 is a subunit of the 26S proteasome; this large, multi-protein complex is a component of the ubiquitin-proteasome pathway, which is involved in a variety of basic cellular processes and inflammatory responses.
  
  Conclusion
  
  To our knowledge, this is the first report that SARS-CoV NP interacts with the proteasome subunit p42 within host cells. These data enhance our understanding of the molecular mechanisms of SARS-CoV pathogenicity and the means by which SARS-CoV interacts with host cells.
  
  
• Subjects:
  Cell Line Host-Pathogen Interactions Humans Nucleocapsid Proteins Proteasome Endopeptidase Complex Protein Binding Protein Transport Research SARS Virus Severe Acute Respiratory Syndrome
• Source:
  Virol J. 2010; 7:99.
• Document Type:
  Journal Article
• Volume:
  7
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:403a781bf845584bfe421cc3bbca7a6185645ab41483292771d684db2702661f
• Download URL:
  https://stacks.cdc.gov/view/cdc/3640/cdc_3640_DS1.pdf
• File Type:
  [PDF - 771.54 KB ]