ICAM-1 regulates the survival of influenza virus in lung epithelial cells during the early stages of infection

Othumpangat, Sreekumar; Noti, John D.; McMillen, Cynthia M.; Beezhold, Donald H.

doi:10.1016/j.virol.2015.10.005

Back to Previous Page

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

ICAM-1 regulates the survival of influenza virus in lung epithelial cells during the early stages of infection

Published Date:

Oct 23 2015

Publisher's site:

http://dx.doi.org/10.1016/j.virol.2015.10.005 New Site Icon

Source:

Virology. 487:85-94.

[PDF-1.65 MB]

Details:

Alternative Title:

Virology

Personal Author:

Othumpangat, Sreekumar ; Noti, John D. ; McMillen, Cynthia M. ; Beezhold, Donald H. ;

Description:

Intercellular cell adhesion molecule-1 (ICAM-1) is an inducible cell surface glycoprotein that is expressed on many cell types. Influenza virus infection enhanced ICAM-1 expression and messenger RNA levels. Human bronchial epithelial cells (HBEpC) and nasal epithelial cells, on exposure to different strains of influenza virus (H1N1, H3N2, and H9N1) showed significant increase in ICAM-1 gene expression (p<0.001) along with the ICAM-1 protein levels (surface and secreted). Depleting ICAM-1 in HBEpC with ICAM-1 siRNA and subsequently infecting with H1N1 showed increased viral copy numbers. Influenza virus infection in HBEpC resulted in up-regulation of NF-ĸB protein and the lack of ICAM-1 decreased NF-ĸB activity in NF-ĸB luciferase reporter assay. Addition of exogenous IL-1β to HBEpC induced the ICAM-1 expression and decreased matrix gene copy number. Taken together, HBEpC induced ICAM-1 plays a key role in modulating the influenza virus survival possibly through the NF-ĸB pathway.

Subject:

[+]

Pubmed ID:

26499045

Pubmed Central ID:

PMC4719144

Document Type:

Journal Article

Funding:

CC999999/Intramural CDC HHS/United States

Collection(s):

CDC Public Access

Main Document Checksum:

[+]

Supporting Files:

	nihms749752f4.jpg	image/jpeg
	nihms749752f5.gif	image/gif
	nihms749752f5.jpg	image/jpeg
	nihms749752f6.gif	image/gif
	nihms749752f6.jpg	image/jpeg
	nihms749752f7.gif	image/gif
	nihms749752f7.jpg	image/jpeg
	nihms749752f8.gif	image/gif
	nihms749752f8.jpg	image/jpeg
	nihms749752f9.gif	image/gif
	nihms749752.nxml	application/octet-stream
	nihms749752f9.jpg	image/jpeg
	nihms749752f1.gif	image/gif
	nihms749752f1.jpg	image/jpeg
	nihms749752f2.gif	image/gif
	nihms749752f2.jpg	image/jpeg
	nihms749752f3.gif	image/gif
	nihms749752f3.jpg	image/jpeg
	nihms749752f4.gif	image/gif

No Related Documents.

You May Also Like:

Lung epithelial cells resist influenza A infection by inducing the expression of cytochrome c oxidase VIc which is modulated by miRNA 4276

Personal Author:

Othumpangat, Sreekumar; Noti, John D.; Beezhold, Donald H.;

Published Date:

Sep 07 2014

Source:

Virology. 0:256-264.

Description:

Influenza virus infection induces several changes in host miRNA profile, host cell death and tissue damage. Cytochrome c is a regulator of the intrinsic apoptotic pathway and is altered during viral infections. Within the first 3h of infection with i...

File Type:

[PDF - 1.08 MB]
Influenza virus infection modulates the death receptor pathway during early stages of infection in human bronchial epithelial cells

Personal Author:

Othumpangat, Sreekumar; Beezhold, Donald H.; Noti, John D.;

Published Date:

June 29 2018

Source:

Physiol Genomics. 50(9):770-779

Description:

Host-viral interaction occurring throughout the infection process between the influenza A virus (IAV) and bronchial cells determines the success of infection. Our previous studies showed that the apoptotic pathway triggered by the host cells was repr...

File Type:

[PDF - 1.84 MB]
The Sources of Inflammatory Mediators in the Lung after Silica Exposure

Personal Author:

Rao, K. Murali Krishna; Porter, Dale W.; Meighan, Terence; Castranova, Vince;

Published Date:

Dec 2004

Source:

Environ Health Perspect. ; 112(17):1679-1685.

Description:

The expression of 10 genes implicated in regulation of the inflammatory processes in the lung was studied after exposure of alveolar macrophages (AMs) to silica in vitro or in vivo. Exposure of AMs to silica in vitro up-regulated the messenger RNA (m...

File Type:

[PDF - 173.79 KB]
Expression of non-structural-1A binding protein in lung epithelial cells is modulated by miRNA-548an on exposure to influenza A virus

Personal Author:

Othumpangat, Sreekumar; Noti, John D.; Blachere, Francoise M.; Beezhold, Donald H.;

Published Date:

Sep 20 2013

Source:

Virology. 447(0):84-94.

Description:

Understanding the host response to influenza A virus infection is essential for developing intervention approaches. We show that infection of human alveolar epithelial cells and human bronchial epithelial cells with influenza A for 3h resulted in dow...

File Type:

[PDF - 1.38 MB]
Dynamic regulation of cardiolipin by the lipid pump, ATP8b1, determines the severity of lung injury in experimental bacterial pneumonia

Personal Author:

Ray, Nancy B.; Durairaj, Lakshmi; Chen, Bill B.; McVerry, Bryan J.; Ryan, Alan J.; Donahoe, Michael; Waltenbaugh, Alisa K.; O’Donnell, Christopher P.; Henderson, Florita C.; Etscheidt, Christopher A.; McCoy, Diann M.; Agassandian, Marianna; Hayes-Rowan, Emily C.; Coon, Tiffany A.; Butler, Phillip L.; Gakhar, Lokesh; Mathur, Satya N.; Sieren, Jessica C.; Tyurina, Yulia Y.; Kagan, Valerian E.; McLennan, Geoffrey; Mallampalli, Rama K.;

Published Date:

Sep 19 2010

Source:

Nat Med. 16(10):1120-1127.

Description:

Pneumonia remains the leading cause of death from infection in the US, yet fundamentally new conceptual models underlying its pathogenesis have not emerged. We show that humans and mice with bacterial pneumonia have markedly elevated amounts of cardi...

File Type:

[PDF - 1.02 MB]