ICAM-1 regulates the survival of influenza virus in lung epithelial cells during the early stages of infection

Othumpangat, Sreekumar; Noti, John D.; McMillen, Cynthia M.; Beezhold, Donald H.

doi:10.1016/j.virol.2015.10.005

i

ICAM-1 regulates the survival of influenza virus in lung epithelial cells during the early stages of infection

Supporting Files

Oct 23 2015
By Othumpangat, Sreekumar ; Noti, John D. ; McMillen, Cynthia M. ; ...

Details

Alternative Title:

Virology
Personal Author:

Othumpangat, Sreekumar ; Noti, John D. ; McMillen, Cynthia M. ; Beezhold, Donald H.
Description:

Intercellular cell adhesion molecule-1 (ICAM-1) is an inducible cell surface glycoprotein that is expressed on many cell types. Influenza virus infection enhanced ICAM-1 expression and messenger RNA levels. Human bronchial epithelial cells (HBEpC) and nasal epithelial cells, on exposure to different strains of influenza virus (H1N1, H3N2, and H9N1) showed significant increase in ICAM-1 gene expression (p<0.001) along with the ICAM-1 protein levels (surface and secreted). Depleting ICAM-1 in HBEpC with ICAM-1 siRNA and subsequently infecting with H1N1 showed increased viral copy numbers. Influenza virus infection in HBEpC resulted in up-regulation of NF-ĸB protein and the lack of ICAM-1 decreased NF-ĸB activity in NF-ĸB luciferase reporter assay. Addition of exogenous IL-1β to HBEpC induced the ICAM-1 expression and decreased matrix gene copy number. Taken together, HBEpC induced ICAM-1 plays a key role in modulating the influenza virus survival possibly through the NF-ĸB pathway.
Subjects:

Animals Article Bronchi Cell Line Dogs Epithelial Cells Extracellular Matrix Gene Expression Humans Influenza A Virus, H1N1 Subtype Influenza A Virus, H3N2 Subtype Influenza Virus Intercellular Adhesion Molecule-1 Intercellular Cell Adhesion Molecule Interleukin-1beta Lung Epithelial Cells Madin Darby Canine Kidney Cells NF-κB Respiratory Mucosa RNA, Small Interfering RNA Interference Signaling Transcription Factor RelA
Source:

Virology. 487:85-94.
Pubmed ID:

26499045
Pubmed Central ID:

PMC4719144
Document Type:

Journal Article
Funding:

CC999999/Intramural CDC HHS/United States
Volume:

487
Collection(s):

CDC Public Access
Main Document Checksum:

urn:sha256:5f6d2e7a5b1458d00a79c225207386631003005b450ba5ec515f0ecc46ec814f
Download URL:

https://stacks.cdc.gov/view/cdc/37653/cdc_37653_DS1.pdf
File Type:

[PDF - 1.65 MB ]

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