Species identification of invasive yeasts including Candida in Pakistan: limitations of phenotypic methods

Details

• Alternative Title:
  J Pak Med Assoc
• Personal Author:
  Farooqi, Joveria ; Jabeen, Kauser ; Saeed, Noureen ; Zafar, Afia ; Brandt, Mary Eleanor ; Hasan, Rumina
• Description:
  Objective
  
  To compare phenotypic and genotypic methods of yeast identification.
  
  Methods
  
  The in-vitro cross-sectional study was conducted from January 2006 to May 2009. Invasive yeasts isolated at the clinical microbiology laboratory at the Aga Khan University (AKU), Karachi, Pakistan, were identified. Speciation by phenotypic and molecular methods was compared. All yeasts isolated during the study period from blood and other invasive sites were identified using standard methods. Isolates were shipped to Mycotic Diseases Branch, Centres for Disease Control and Prevention, Atlanta, Georgia, USA, for identification by Luminex flow cytometric multianalyte profiling (xMAP) system. Ribosomal ITS2 DNA sequencing was performed on isolates not identified by Luminex.
  
  Result
  
  Of the 214 invasive yeasts evaluated, Candida species were 209 (97.7%) while the frequency of non-Candida species was 5 (2.3%). Overall agreement between phenotypic and molecular identification was 81.3%, 90.3% amongst the more common Candida species, and only 38.8% amongst the uncommon yeasts.
  
  Conclusion
  
  Phenotypic methods of identification proved adequate for common Candida species, but were deficient in recognising rare Candida and non-Candida yeasts, highlighting the importance of molecular methods for identification.
  
  
• Subjects:
  Candida Cross-Sectional Studies Flow Cytometry Genotype Humans In Vitro Techniques Mycological Typing Techniques Mycoses Pakistan Phenotype Polymerase Chain Reaction Yeasts

  More +
• Keywords:
  Cryptococcus Invasive Yeasts Luminex MAP Rhodotorula
• Source:
  J Pak Med Assoc. 62(10):995-998
• Pubmed ID:
  23866432
• Pubmed Central ID:
  PMC4624294
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHSUnited States/
• Volume:
  62
• Issue:
  10
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:6762c6666771b17d3c70afc06c3e6d9dd2d324bdcbde21f34054658f33320734c86a53fd0fac17a49dff11309bc143f9780fac75dd1d20804f41f75a56decfdc
• Download URL:
  https://stacks.cdc.gov/view/cdc/36308/cdc_36308_DS1.pdf
• File Type:
  [PDF - 141.25 KB ]