A real-time PCR assay for detection of the Ehrlichia muris-like agent, a newly recognized pathogen of humans in the upper Midwestern United States

Allerdice, Michelle E.J.; Pritt, Bobbi S.; Sloan, Lynne M.; Paddock, Christopher D.; Karpathy, Sandor E.

doi:10.1016/j.ttbdis.2015.10.004

- Add this to...
- Twitter
- Facebook
- Google Bookmarks
- LinkedIn

A real-time PCR assay for detection of the Ehrlichia muris-like agent, a newly recognized pathogen of humans in the upper Midwestern United States

Published Date:

Oct 22 2015

Publisher's site:

http://dx.doi.org/10.1016/j.ttbdis.2015.10.004 New Site Icon

Source:

Ticks Tick Borne Dis. 7(1):146-149.

[PDF-284.76 KB]

Details:

Personal Authors:

Allerdice, Michelle E.J. ; Pritt, Bobbi S. ; Sloan, Lynne M. ; Paddock, Christopher D. ; Karpathy, Sandor E. ;

Keywords:

[+]

Pubmed ID:

26507653

Pubmed Central ID:

PMC4659714

Description:

The Ehrlichia muris-like agent (EMLA) is an emerging, tick-transmitted human pathogen that occurs in the upper Midwestern United States. Here, we describe the development and validation of a p13-based quantitative real-time PCR TaqMan assay to detect EMLA in blood or tissues of ticks, humans, and rodents. The primer and probe specificities of the assay were ascertained using a large panel of various Ehrlichia species and other members of Rickettsiales. In addition to control DNA, both non-infected and EMLA-infected human blood, Mus musculus blood, and M. musculus tissue extracts were evaluated, as were non-infected and EMLA-infected Ixodes scapularis and uninfected Dermacentor variabilis DNA lysates. The specificity of the probe was determined via real-time PCR. An EMLA p13 control plasmid was constructed, and serial dilutions were used to determine the analytical sensitivity, which was found to be 1 copy per 4μl of template DNA. The sensitivity and specificity of this assay provides a powerful tool for ecological studies involving arthropod vectors and their mammalian hosts.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

CC999999/Intramural CDC HHS/United States

Main Document Checksum:

[+]

Supporting Files:

	nihms-732087-f0001.gif	image/gif
	nihms-732087-f0001.jpg	image/jpeg
	nihms-732087-f0002.gif	image/gif
	nihms-732087-f0002.jpg	image/jpeg
	nihms-732087.nxml	application/octet-stream

No Related Documents.

You May Also Like:

Ehrlichia muris in Ixodes cookei Ticks, Northeastern United States, 2016–2017

Personal Author:

Xu, Guang; Pearson, Patrick; Rich, Stephen M.;

Published:

Jun 2018

Source:

Emerg Infect Dis. 24(6):1143-1144.

Description:

Ehrlichia muris is an agent of human ehrlichiosis. To determine its geographic spread in the United States, during 2016-2017, we tested 8,760 ticks from 45 states. A distinct clade of E. muris found in 3 Ixodes cookei ticks from the northeastern Unit...

File Type:

[PDF - 427.92 KB]
Co-Feeding Transmission of the Ehrlichia muris–Like Agent to Mice (Mus musculus)

Personal Author:

Karpathy, Sandor E.; Allerdice, Michelle E.J.; Sheth, Mili; Dasch, Gregory A.; Levin, Michael L.;

Published:

Jan 29 2016

Source:

Vector Borne Zoonotic Dis. 16(3):145-150.

Description:

The Ehrlichia muris-like agent (EMLA) is a newly recognized human pathogen found in Wisconsin and Minnesota. Ecological investigations have implicated both the blacklegged tick, Ixodes scapularis, and the white-footed mouse, Peromyscus leucopus, as p...

File Type:

[PDF - 297.50 KB]
Surveillance for Ixodes scapularis and pathogens found in this tick species in the United States

Corporate Author:

National Center for Emerging and Zoonotic Infectious Diseases (U.S.). Division of Vector-Borne Diseases. Bacterial Diseases Branch.

Published:

02/22/2019

Description:

Public health entomologists/biologists are the intended audience for this document. : The primary objective of this document is to provide guidance for surveillance of the western blacklegged tick, Ixodes pacificus, and pathogens found in this tick s...

File Type:

[PDF - 1.13 MB]
Prevalence and distribution of seven human pathogens in host-seeking Ixodes scapularis (Acari: Ixodidae) nymphs in Minnesota, USA

Personal Author:

Johnson, Tammi L.; Graham, Christine B.; Maes, Sarah E.; Hojgaard, Andrias; Fleshman, Amy; Boegler, Karen A.; Delory, Mark J.; Slater, Kimetha S.; Karpathy, Sandor E.; Bjork, Jenna K.; Neitzel, David F.; Schiffman, Elizabeth K.; Eisen, Rebecca J.;

Published:

July 20 2018

Source:

Ticks Tick Borne Dis. 9(6):1499-1507

Description:

In the north-central United States, the blacklegged tick (Ixodes scapularis) is currently known to vector seven human pathogens. These include five bacteria (Borrelia burgdorferi sensu stricto, Borrelia mayonii, Borrelia miyamotoi, Anaplasma phagocyt...

File Type:

[PDF - 1.48 MB]
Human Infection with Ehrlichia muris–like Pathogen, United States, 2007–20131

Personal Author:

Johnson, Diep K. Hoang; Schiffman, Elizabeth K.; Davis, Jeffrey P.; Neitzel, David F.; Sloan, Lynne M.; Nicholson, William L.; Fritsche, Thomas R.; Steward, Christopher R.; Ray, Julie A.; Miller, Tracy K.; Feist, Michelle A.; Uphoff, Timothy S.; Franson, Joni J.; Livermore, Amy L.; Deedon, Alecia K.; Theel, Elitza S.; Pritt, Bobbi S.;

Published:

Oct 2015

Source:

Emerg Infect Dis. 21(10):1794-1799

Description:

An Ehrlichia muris-like (EML) pathogen was detected among 4 patients in Minnesota and Wisconsin during 2009. We characterized additional cases clinically and epidemiologically. During 2004-2013, blood samples from 75,077 patients from all 50 United S...

File Type:

[PDF - 448.66 KB]