Effect of time at temperature on wild poliovirus titers in stool specimens

Details

• Alternative Title:
  Virology
• Personal Author:
  Walker, Allison Taylor ; Williams, Alford J. ; Gary, Howard E. ; Pallansch, Mark A. ; Wassilak, Steven G.F. ; Oberste, M. Steven
• Description:
  Background
  
  The effect of transport temperature on the viability of poliovirus in stool specimens from paralyzed cases has not been tested. Quality assurance of programmatic indicators will be necessary in the final phase of polio eradication.
  
  Objective
  
  To estimate the effect of time at elevated temperatures on wild poliovirus titers in stool specimens.
  
  Methods
  
  We exposed aliquots of pooled wild poliovirus type 1 specimens to elevated temperatures (27 °C, 31 °C, and 35 °C) for varying time periods up to 14 days. We determined the virus titer of these aliquots and created decay curves at each temperature to estimate the relationship between time at temperature and virus titer.
  
  Results
  
  We found significantly different slopes of decay at each temperature. The negative slopes increased as the temperature increased.
  
  Conclusions
  
  While poliovirus in stool remains relatively stable at moderately elevated temperature, transport at higher temperatures could impact sample integrity and virus isolation results.
  
  
• Subjects:
  Article Feces Humans Microbial Viability Polio Poliovirus Quality Assurance Reverse Cold Chain Specimen Handling Specimen Transport Temperature Time Factors Viral Load

  More +
• Source:
  Virology. 482:28-31.
• Pubmed ID:
  25817402
• Pubmed Central ID:
  PMC4620922
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHS/United States
• Volume:
  482
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:753144760472101fc60368bbb0e4daa0572534987d816bce3e6c5547f09a1921
• Download URL:
  https://stacks.cdc.gov/view/cdc/35362/cdc_35362_DS1.pdf
• File Type:
  [PDF - 344.03 KB ]