Development of an Immunoassay for the Detection of the Phenylpyrazole Insecticide Fipronil

Vasylieva, Natalia; Ahn, Ki Chang; Barnych, Bogdan; Gee, Shirley J.; Hammock, Bruce D.

doi:10.1021/acs.est.5b01005

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CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners. As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.

i

Development of an Immunoassay for the Detection of the Phenylpyrazole Insecticide Fipronil

Aug 05 2015
By Vasylieva, Natalia ; Ahn, Ki Chang ; Barnych, Bogdan ; ...
http://dx.doi.org/10.1021/acs.est.5b01005
Source: Environ Sci Technol. 49(16):10038-10047.

[PDF-896.96 KB]

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Details:

Alternative Title:

Environ Sci Technol
Personal Author:

Vasylieva, Natalia ; Ahn, Ki Chang ; Barnych, Bogdan ; Gee, Shirley J. ; Hammock, Bruce D.

Vasylieva, Natalia ; Ahn, Ki Chang ; Barnych, Bogdan ; Gee, Shirley J. ; Hammock, Bruce D. Less -
Description:

Phenylpyrazole insecticides such as fipronil have been used as replacements for organophosphates. The wide application of fipronil raises concern about environmental contamination and risk for fish, birds, and other nontargeted beings as well as human health. A sensitive, competitive indirect heterologous enzyme-linked immunosorbent assay (ELISA) was developed. Antibodies with different specificities to fipronil and its metabolites were produced. Two ELISAs having IC50 values of 0.58 ± 0.06 and 2.6 ± 0.4 ng/mL were developed. Design of different haptens and coating antigens resulted in two assays with distinct cross-reactivity patterns for structurally related compounds: 96, 38, and 101% versus 39, 1.4, and 25% for fipronil-sulfide, fipronil-detrifluoromethylsulfonyl, and fipronil-desulfinyl, respectively. Performance of the immunoassays was demonstrated by a recovery study from spiked water and human serum and urine matrices, giving recovery values in the range of 85-111% for different concentrations. The assays demonstrated good correlation in fipronil recovery with conventional LC-MS/MS analysis. The generic assay 2265 has the sensitivity to measure fipronil and its analogs in serum at levels relevant for exposure monitoring. The assays were used to analyze human urine samples obtained from exposure studies and serum samples from rats treated with a fipronil-containing diet.
Subjects:

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Animals Antibodies Antigens Article Chromatography, Liquid Cross-reactivity Cross Reactions ELISA Environmental Contamination Enzyme-Linked Immunosorbent Assay Fipronil GABA Inhibitor Haptens Human Health Humans Immune Sera Immunoassay Insecticides Male Polyclonal Antibody Pyrazoles Rats Reproducibility Of Results Tandem Mass Spectrometry Water
Source:

Environ Sci Technol. 49(16):10038-10047.
Pubmed ID:

26196357
Pubmed Central ID:

PMC4605820
Document Type:

Journal Article
Funding:

P42 ES004699/ES/NIEHS NIH HHS/United States ; P42 ES04699/ES/NIEHS NIH HHS/United States ; U50 OH07550/OH/NIOSH CDC HHS/United States ; U54 NS079202/NS/NINDS NIH HHS/United States ; U54 NS079202/NS/NINDS NIH HHS/United States

P42 ES004699/ES/NIEHS NIH HHS/United States ; P42 ES04699/ES/NIEHS NIH HHS/United States ; U50 OH07550/OH/NIOSH CDC HHS/United States ; U54 NS079202/NS/NINDS NIH HHS/United States ; U54 NS079202/NS/NINDS NIH HHS/United States Less -
Volume:

49
Issue:

16
Collection(s):

CDC Public Access
Main Document Checksum:

[+]

urn:sha256:51093fc4aeb67be75f1c85b5a4ce3e15bb0fc2e420c2003ec082f02aac618df2
Download URL:

https://stacks.cdc.gov/view/cdc/35274/cdc_35274_DS1.pdf
File Type:

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