Genetic analysis of G12P[8] rotaviruses detected in the largest U.S. G12 genotype outbreak on record

Mijatovic-Rustempasic, Slavica; Teel, Elizabeth N.; Kerin, Tara K.; Hull, Jennifer; Roy, Sunando; Weinberg, Geoffrey A.; Payne, Daniel C.; Parashar, Umesh D.; Gentsch, Jon R.; Bowen, Michael D.

doi:10.1016/j.meegid.2013.11.004

i

Genetic analysis of G12P[8] rotaviruses detected in the largest U.S. G12 genotype outbreak on record

Supporting Files

Nov 21 2013
By Mijatovic-Rustempasic, Slavica ; Teel, Elizabeth N. ; Kerin, Tara K. ; ...

File Language:

English

Details

Alternative Title:

Infect Genet Evol
Personal Author:

Mijatovic-Rustempasic, Slavica ; Teel, Elizabeth N. ; Kerin, Tara K. ; Hull, Jennifer ; Roy, Sunando ; Weinberg, Geoffrey A. ; Payne, Daniel C. ; Parashar, Umesh D. ; Gentsch, Jon R. ; Bowen, Michael D.
Description:

In 2006-07, 77 cases of gastroenteritis in Rochester, NY, USA were associated with rotavirus genotype G12P[8]. Sequence analysis identified a high degree of genetic relatedness among the VP7 and VP4 genes of the Rochester G12P[8] strains and between these strains and currently circulating human G12P[8] strains. Out of 77 samples, two and seven unique nucleotide sequences were identified for VP7 and VP4 genes, respectively. Rochester strain VP7 genes were found to occupy the G12-III lineage and VP4 genes clustered within the P[8]-3 lineage. Six strains contained non-synonymous nucleotide substitutions that produced amino acid changes at 6 sites in the VP8(∗) region of the VP4 gene. Two sites (amino acids 242 and 246) were located in or near a described trypsin cleavage site. Selection analyses identified one positively selected VP7 site (107) and strong purifying selection at 58 sites within the VP7 gene as well as 2 of the 6 variant sites (79 and 218) in VP4.
Subjects:

Antigens, Viral Capsid Proteins Disease Outbreaks Evolution, Molecular Gastroenteritis Genetic Variation Genome, Viral Genotype Humans Phylogeny Polymorphism, Genetic Rotavirus Rotavirus Infections Rotavirus Vaccines
Source:

Infect Genet Evol. 21:214-219.
Pubmed ID:

24270016
Pubmed Central ID:

PMC4612336
Document Type:

Journal Article
Funding:

CC999999/Intramural CDC HHS/United States
Place as Subject:

United States
Volume:

21
Collection(s):

CDC Public Access
Main Document Checksum:

urn:sha256:79c909379e2726c49fd2f36acd741e63f263af93aa063ea0126229d30bce7875
Download URL:

https://stacks.cdc.gov/view/cdc/35196/cdc_35196_DS1.pdf
File Type:

[PDF - 1.89 MB ]

File Language:

English

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