Details:

Alternative Title:
Malar J
Personal Author:
Lucchi, Naomi W ; Tongren, Jon Eric ; Jain, Vidhan ; Nagpal, Avinash C ; Kauth, Christian W ; ... More +
Lucchi, Naomi W ; Tongren, Jon Eric ; Jain, Vidhan ; Nagpal, Avinash C ; Kauth, Christian W ; Woehlbier, Ute ; Bujard, Hermann ; Dash, Aditya P ; Singh, Neeru ; Stiles, Jonathan K ; Udhayakumar, Venkatachalam Less -
Description:
Background

Plasmodium falciparum infection causes cerebral malaria (CM) in a subset of patients with anti-malarial treatment protecting only about 70% to 80% of patients. Why a subset of malaria patients develops CM complications, including neurological sequelae or death, is still not well understood. It is believed that host immune factors may modulate CM outcomes and there is substantial evidence that cellular immune factors, such as cytokines, play an important role in this process. In this study, the potential relationship between the antibody responses to the merozoite surface protein (MSP)-1 complex (which consists of four fragments namely: MSP-183, MSP-130, MSP-138 and MSP-142), MSP-636 and MSP-722 and CM was investigated.

Methods

Peripheral blood antibody responses to recombinant antigens of the two major allelic forms of MSP-1 complex, MSP-636 and MSP-722 were compared between healthy subjects, mild malaria patients (MM) and CM patients residing in a malaria endemic region of central India. Total IgG and IgG subclass antibody responses were determined using ELISA method.

Results

The prevalence and levels of IgG and its subclasses in the plasma varied for each antigen. In general, the prevalence of total IgG, IgG1 and IgG3 was higher in the MM patients and lower in CM patients compared to healthy controls. Significantly lower levels of total IgG antibodies to the MSP-1f38, IgG1 levels to MSP-1d83, MSP-119 and MSP-636 and IgG3 levels to MSP-1f42 and MSP-722 were observed in CM patients as compared to MM patients.

Conclusion

These results suggest that there may be some dysregulation in the generation of antibody responses to some MSP antigens in CM patients and it is worth investigating further whether perturbations of antibody responses in CM patients contribute to pathogenesis.


Subjects:
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Adult Animals Antibodies, Protozoan Antigens, Protozoan Enzyme-Linked Immunosorbent Assay Female Humans Immunoglobulin G Malaria, Cerebral Malaria, Falciparum Male Merozoite Surface Protein 1 Plasmodium Falciparum Recombinant Proteins Research
Source:
Malar J. 2008; 7:121.
Document Type:
Journal Article
Place as Subject:
India
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:7bcd3ec4997849e2ecc64a89f06f978305b82beb523848236d1d7fcaa5fbed90
Download URL:
https://stacks.cdc.gov/view/cdc/3499/cdc_3499_DS1.pdf
File Type:

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